CIDEA interacts with liver X receptors in white fat cells

Agné Kulyté; Amanda T. Pettersson; Per Antonson; Britta M. Stenson; Dominique Langin; Jan Ke Gustafsson; Bart Staels; Mikael Rydén; Peter Arner; Jurga Laurencikiene

doi:10.1016/j.febslet.2011.02.004

CIDEA interacts with liver X receptors in white fat cells

Agné Kulyté, Amanda T. Pettersson, Per Antonson, Britta M. Stenson, Dominique Langin, Jan Ke Gustafsson, Bart Staels, Mikael Rydén, Peter Arner, Jurga Laurencikiene

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Cell death-inducing DNA fragmentation factor alpha-like effector A (CIDEA) is endogenously expressed in human but not rodent white adipocytes. We performed a bioinformatic analysis of the human CIDEA sequence and found conserved amino-acid motifs involved in binding to nuclear receptors. Protein-protein binding experiments and transactivation assays confirmed that CIDEA binds to liver X receptors and regulates their activity in vitro. Cell fractionation demonstrated that CIDEA localizes to both the cytoplasm and the nucleus in human white adipocytes. The interaction between CIDEA and nuclear receptors could therefore be of importance for the regulation of metabolic processes in human adipose tissue. Structured summary: LXR-beta binds to CIDEA by pull down (View interaction) TIF2 and LXR-beta physically interact by two hybrid (View interaction) LXR-beta binds to RAP250 by pull down (View interaction) CIDEA and LXR-beta physically interact by two hybrid (View interaction)

Original language	English (US)
Pages (from-to)	744-748
Number of pages	5
Journal	FEBS Letters
Volume	585
Issue number	5
DOIs	https://doi.org/10.1016/j.febslet.2011.02.004
State	Published - Mar 9 2011

Keywords

Adipocytes
Lipid metabolism
Nuclear receptors

ASJC Scopus subject areas

Biochemistry
Biophysics
Cell Biology
Genetics
Molecular Biology
Structural Biology

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10.1016/j.febslet.2011.02.004

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title = "CIDEA interacts with liver X receptors in white fat cells",

abstract = "Cell death-inducing DNA fragmentation factor alpha-like effector A (CIDEA) is endogenously expressed in human but not rodent white adipocytes. We performed a bioinformatic analysis of the human CIDEA sequence and found conserved amino-acid motifs involved in binding to nuclear receptors. Protein-protein binding experiments and transactivation assays confirmed that CIDEA binds to liver X receptors and regulates their activity in vitro. Cell fractionation demonstrated that CIDEA localizes to both the cytoplasm and the nucleus in human white adipocytes. The interaction between CIDEA and nuclear receptors could therefore be of importance for the regulation of metabolic processes in human adipose tissue. Structured summary: LXR-beta binds to CIDEA by pull down (View interaction) TIF2 and LXR-beta physically interact by two hybrid (View interaction) LXR-beta binds to RAP250 by pull down (View interaction) CIDEA and LXR-beta physically interact by two hybrid (View interaction)",

keywords = "Adipocytes, Lipid metabolism, Nuclear receptors",

author = "Agn{\'e} Kulyt{\'e} and Pettersson, {Amanda T.} and Per Antonson and Stenson, {Britta M.} and Dominique Langin and Gustafsson, {Jan Ke} and Bart Staels and Mikael Ryd{\'e}n and Peter Arner and Jurga Laurencikiene",

note = "Funding Information: The excellent technical assistance of Gaby {\AA}str{\"o}m, Eva Sjolin and Kerstin Wahlen is appreciated. This study was supported by grants from the Swedish Research Council 522-20082900 , NovoNordisk , {\AA}ke Wiberg , Magnus Bergvall Foundations , Karolinska Institutet and the European Union ( HEPADIP , LSHM-CT-2005-018734 , COST BM0602 , ADAPT HEALTH-F2-2008-201100 ).",

year = "2011",

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doi = "10.1016/j.febslet.2011.02.004",

language = "English (US)",

volume = "585",

pages = "744--748",

journal = "FEBS Letters",

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T1 - CIDEA interacts with liver X receptors in white fat cells

AU - Kulyté, Agné

AU - Pettersson, Amanda T.

AU - Antonson, Per

AU - Stenson, Britta M.

AU - Langin, Dominique

AU - Gustafsson, Jan Ke

AU - Staels, Bart

AU - Rydén, Mikael

AU - Arner, Peter

AU - Laurencikiene, Jurga

N1 - Funding Information: The excellent technical assistance of Gaby Åström, Eva Sjolin and Kerstin Wahlen is appreciated. This study was supported by grants from the Swedish Research Council 522-20082900 , NovoNordisk , Åke Wiberg , Magnus Bergvall Foundations , Karolinska Institutet and the European Union ( HEPADIP , LSHM-CT-2005-018734 , COST BM0602 , ADAPT HEALTH-F2-2008-201100 ).

PY - 2011/3/9

Y1 - 2011/3/9

N2 - Cell death-inducing DNA fragmentation factor alpha-like effector A (CIDEA) is endogenously expressed in human but not rodent white adipocytes. We performed a bioinformatic analysis of the human CIDEA sequence and found conserved amino-acid motifs involved in binding to nuclear receptors. Protein-protein binding experiments and transactivation assays confirmed that CIDEA binds to liver X receptors and regulates their activity in vitro. Cell fractionation demonstrated that CIDEA localizes to both the cytoplasm and the nucleus in human white adipocytes. The interaction between CIDEA and nuclear receptors could therefore be of importance for the regulation of metabolic processes in human adipose tissue. Structured summary: LXR-beta binds to CIDEA by pull down (View interaction) TIF2 and LXR-beta physically interact by two hybrid (View interaction) LXR-beta binds to RAP250 by pull down (View interaction) CIDEA and LXR-beta physically interact by two hybrid (View interaction)

AB - Cell death-inducing DNA fragmentation factor alpha-like effector A (CIDEA) is endogenously expressed in human but not rodent white adipocytes. We performed a bioinformatic analysis of the human CIDEA sequence and found conserved amino-acid motifs involved in binding to nuclear receptors. Protein-protein binding experiments and transactivation assays confirmed that CIDEA binds to liver X receptors and regulates their activity in vitro. Cell fractionation demonstrated that CIDEA localizes to both the cytoplasm and the nucleus in human white adipocytes. The interaction between CIDEA and nuclear receptors could therefore be of importance for the regulation of metabolic processes in human adipose tissue. Structured summary: LXR-beta binds to CIDEA by pull down (View interaction) TIF2 and LXR-beta physically interact by two hybrid (View interaction) LXR-beta binds to RAP250 by pull down (View interaction) CIDEA and LXR-beta physically interact by two hybrid (View interaction)

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KW - Lipid metabolism

KW - Nuclear receptors

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CIDEA interacts with liver X receptors in white fat cells

Abstract

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