Chromosome mapping of the murine syndecan gene

Henry F. Oettinger; Heather Streeter; Edward Lose; Neal G. Copeland; Debra J. Gilbert; Monica J. Justice; Nancy A. Jenkins; T. Mohandas; Merton Bernfield

doi:10.1016/0888-7543(91)90140-A

Chromosome mapping of the murine syndecan gene

Henry F. Oettinger, Heather Streeter, Edward Lose, Neal G. Copeland, Debra J. Gilbert, Monica J. Justice, Nancy A. Jenkins, T. Mohandas, Merton Bernfield

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Abstract

The chromosomal localization of the murine syndecan gene was determined by analysis of DNA from a panel of mouse-hamster cell hybrids containing various mouse chromosomes, detection of immunoreactive syndecan in culture medium of these cells, and linkage analysis of a mouse interspecific backcross. Southern analysis of the mouse-hamster cell hybrid DNA shows two distinct hybridizing sequences, one on mouse Chromosome 12 and the other on the X chromosome. Localization of the syndecan gene to mouse Chromosome 12 was determined by detection of immunoreactive syndecan in the culture medium of cell hybrids containing mouse Chromosome 12. Hybrids containing other mouse chromosomes were negative. Linkage analysis by Southern hybridization of DNA from a mouse interspecific backcross using a syndecan-specific probe localized the syndecan gene locus, Synd, to the proximal end of Chromosome 12, tightly linked to the Pomc-1 and Nmyc loci. The syndecan gene is likely on human Chromosome 2 because this region shows conservation of synteny between mouse and human chromosomes.

Original language	English (US)
Pages (from-to)	334-338
Number of pages	5
Journal	Genomics
Volume	11
Issue number	2
DOIs	https://doi.org/10.1016/0888-7543(91)90140-A
State	Published - Oct 1991

ASJC Scopus subject areas

Genetics

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title = "Chromosome mapping of the murine syndecan gene",

abstract = "The chromosomal localization of the murine syndecan gene was determined by analysis of DNA from a panel of mouse-hamster cell hybrids containing various mouse chromosomes, detection of immunoreactive syndecan in culture medium of these cells, and linkage analysis of a mouse interspecific backcross. Southern analysis of the mouse-hamster cell hybrid DNA shows two distinct hybridizing sequences, one on mouse Chromosome 12 and the other on the X chromosome. Localization of the syndecan gene to mouse Chromosome 12 was determined by detection of immunoreactive syndecan in the culture medium of cell hybrids containing mouse Chromosome 12. Hybrids containing other mouse chromosomes were negative. Linkage analysis by Southern hybridization of DNA from a mouse interspecific backcross using a syndecan-specific probe localized the syndecan gene locus, Synd, to the proximal end of Chromosome 12, tightly linked to the Pomc-1 and Nmyc loci. The syndecan gene is likely on human Chromosome 2 because this region shows conservation of synteny between mouse and human chromosomes.",

author = "Oettinger, {Henry F.} and Heather Streeter and Edward Lose and Copeland, {Neal G.} and Gilbert, {Debra J.} and Justice, {Monica J.} and Jenkins, {Nancy A.} and T. Mohandas and Merton Bernfield",

note = "Funding Information: We thank P. Neumann for critically reading the manuscript, B. Stanton and L. Parada for the Nmyc probe, and D. Swing and B. Cho for excellent technical assistance. This research was supported in part by the Lucille P. Markey Charitable Trust (M.B.) and the National Cancer Institute, DHHS, under Grant CA28735 to M.B. and under Contract NOl-CO-74101 with ABL. M.J.J. is the recipient of NRSA Postdoctoral Fellowship lF32CAO8853. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1991",

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doi = "10.1016/0888-7543(91)90140-A",

language = "English (US)",

volume = "11",

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AU - Oettinger, Henry F.

AU - Streeter, Heather

AU - Lose, Edward

AU - Copeland, Neal G.

AU - Gilbert, Debra J.

AU - Justice, Monica J.

AU - Jenkins, Nancy A.

AU - Mohandas, T.

AU - Bernfield, Merton

N1 - Funding Information: We thank P. Neumann for critically reading the manuscript, B. Stanton and L. Parada for the Nmyc probe, and D. Swing and B. Cho for excellent technical assistance. This research was supported in part by the Lucille P. Markey Charitable Trust (M.B.) and the National Cancer Institute, DHHS, under Grant CA28735 to M.B. and under Contract NOl-CO-74101 with ABL. M.J.J. is the recipient of NRSA Postdoctoral Fellowship lF32CAO8853. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1991/10

Y1 - 1991/10

N2 - The chromosomal localization of the murine syndecan gene was determined by analysis of DNA from a panel of mouse-hamster cell hybrids containing various mouse chromosomes, detection of immunoreactive syndecan in culture medium of these cells, and linkage analysis of a mouse interspecific backcross. Southern analysis of the mouse-hamster cell hybrid DNA shows two distinct hybridizing sequences, one on mouse Chromosome 12 and the other on the X chromosome. Localization of the syndecan gene to mouse Chromosome 12 was determined by detection of immunoreactive syndecan in the culture medium of cell hybrids containing mouse Chromosome 12. Hybrids containing other mouse chromosomes were negative. Linkage analysis by Southern hybridization of DNA from a mouse interspecific backcross using a syndecan-specific probe localized the syndecan gene locus, Synd, to the proximal end of Chromosome 12, tightly linked to the Pomc-1 and Nmyc loci. The syndecan gene is likely on human Chromosome 2 because this region shows conservation of synteny between mouse and human chromosomes.

AB - The chromosomal localization of the murine syndecan gene was determined by analysis of DNA from a panel of mouse-hamster cell hybrids containing various mouse chromosomes, detection of immunoreactive syndecan in culture medium of these cells, and linkage analysis of a mouse interspecific backcross. Southern analysis of the mouse-hamster cell hybrid DNA shows two distinct hybridizing sequences, one on mouse Chromosome 12 and the other on the X chromosome. Localization of the syndecan gene to mouse Chromosome 12 was determined by detection of immunoreactive syndecan in the culture medium of cell hybrids containing mouse Chromosome 12. Hybrids containing other mouse chromosomes were negative. Linkage analysis by Southern hybridization of DNA from a mouse interspecific backcross using a syndecan-specific probe localized the syndecan gene locus, Synd, to the proximal end of Chromosome 12, tightly linked to the Pomc-1 and Nmyc loci. The syndecan gene is likely on human Chromosome 2 because this region shows conservation of synteny between mouse and human chromosomes.

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Chromosome mapping of the murine syndecan gene

Abstract

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