Chromosome mapping of the murine syndecan gene

Henry F. Oettinger, Heather Streeter, Edward Lose, Neal G. Copeland, Debra J. Gilbert, Monica J. Justice, Nancy A. Jenkins, T. Mohandas, Merton Bernfield

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

The chromosomal localization of the murine syndecan gene was determined by analysis of DNA from a panel of mouse-hamster cell hybrids containing various mouse chromosomes, detection of immunoreactive syndecan in culture medium of these cells, and linkage analysis of a mouse interspecific backcross. Southern analysis of the mouse-hamster cell hybrid DNA shows two distinct hybridizing sequences, one on mouse Chromosome 12 and the other on the X chromosome. Localization of the syndecan gene to mouse Chromosome 12 was determined by detection of immunoreactive syndecan in the culture medium of cell hybrids containing mouse Chromosome 12. Hybrids containing other mouse chromosomes were negative. Linkage analysis by Southern hybridization of DNA from a mouse interspecific backcross using a syndecan-specific probe localized the syndecan gene locus, Synd, to the proximal end of Chromosome 12, tightly linked to the Pomc-1 and Nmyc loci. The syndecan gene is likely on human Chromosome 2 because this region shows conservation of synteny between mouse and human chromosomes.

Original languageEnglish (US)
Pages (from-to)334-338
Number of pages5
JournalGenomics
Volume11
Issue number2
DOIs
StatePublished - Oct 1991

ASJC Scopus subject areas

  • Genetics

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