Chromosomal location of Evi-1, a common site of ecotropic viral integration of AKXD murine myeloid tumors

The chromosomal location of Evi-1, a common site of ecotropic viral integration in AKXD murine myelogenous leukemias, was determined by recombinant inbred and conventional backcross analyses. We mapped Evi-1 to a location approximately 15 cM distal to the carbonic anhydrase locus on murine Chromosome 3. The chromosomal location of the proto-oncogene Nras, and two growth factors, epidermal growth factor (Egf), and the beta subunit of nerve growth factor (Ngfb), which had previously been assigned to Chromosome 3 by somatic cell hybrid analysis, were also determined. The location of Evi-1 is distinct from these three loci and from all other proto-oncogenes, common sites of viral integration, or growth factor loci previously mapped in mouse chromosomes. These results suggest that Evi-1 represents a new locus involved in myeloid disease.