Chromosomal assignment and genomic structure of Il15

Dirk M. Anderson; Lisabeth Johnson; Moira B. Glaccum; Neal G. Copeland; Debra J. Gilbert; Nancy A. Jenkins; Virginia Valentine; Mark N. Kirstein; David N. Shapiro; Stephan W. Morris; Kenneth Grabstein; David Cosman

doi:10.1016/0888-7543(95)80013-C

Chromosomal assignment and genomic structure of Il15

Dirk M. Anderson, Lisabeth Johnson, Moira B. Glaccum, Neal G. Copeland, Debra J. Gilbert, Nancy A. Jenkins, Virginia Valentine, Mark N. Kirstein, David N. Shapiro, Stephan W. Morris, Kenneth Grabstein, David Cosman

Research output: Contribution to journal › Article › peer-review

108 Scopus citations

Abstract

Interleukin-15 (IL-15) is a novel cytokine whose effects on T-cell activation and proliferation are similar to those of interleukin-2 (IL-2), presumably because IL-15 utilizes the β and γ chains of the IL-2 receptor. Murine IL-15 cDNA and genomic clones were isolated and characterized. The murine Il15 gene was found to consist of eight exons spanning at least 34 kb and was localized to the central region of mouse chromosome 8 by interspecific backcross analysis. Intron positions in a partial human IL15 genomic clone were identical with positions of corresponding introns in the murine gene. The human IL15 gene was mapped to human chromosome 4q31 by fluorescence in situ hybridization.

Original language	English (US)
Pages (from-to)	701-706
Number of pages	6
Journal	Genomics
Volume	25
Issue number	3
DOIs	https://doi.org/10.1016/0888-7543(95)80013-C
State	Published - 1995

ASJC Scopus subject areas

Genetics

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10.1016/0888-7543(95)80013-C

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@article{26a8e92c757143929f14065f7b26733c,

title = "Chromosomal assignment and genomic structure of Il15",

abstract = "Interleukin-15 (IL-15) is a novel cytokine whose effects on T-cell activation and proliferation are similar to those of interleukin-2 (IL-2), presumably because IL-15 utilizes the β and γ chains of the IL-2 receptor. Murine IL-15 cDNA and genomic clones were isolated and characterized. The murine Il15 gene was found to consist of eight exons spanning at least 34 kb and was localized to the central region of mouse chromosome 8 by interspecific backcross analysis. Intron positions in a partial human IL15 genomic clone were identical with positions of corresponding introns in the murine gene. The human IL15 gene was mapped to human chromosome 4q31 by fluorescence in situ hybridization.",

author = "Anderson, {Dirk M.} and Lisabeth Johnson and Glaccum, {Moira B.} and Copeland, {Neal G.} and Gilbert, {Debra J.} and Jenkins, {Nancy A.} and Virginia Valentine and Kirstein, {Mark N.} and Shapiro, {David N.} and Morris, {Stephan W.} and Kenneth Grabstein and David Cosman",

note = "Funding Information: The authors thank Debbie Barnhart for excellent technical assistance and Anthony Namen for the murine fetal liver epithelial cell line. This research was supported, in part, by the National Cancer Institute (NCI), DHHS, under Contract NOl-CO-74101, NC1 Grant CA01702 (S.W.M.), by Cancer Center CORE Grants CA21765 and CA23099 from the NCI, and by the American Lebanese Syrian Associated Charities (ALSAC).",

year = "1995",

doi = "10.1016/0888-7543(95)80013-C",

language = "English (US)",

volume = "25",

pages = "701--706",

journal = "Genomics",

issn = "0888-7543",

publisher = "Academic Press",

number = "3",

}

TY - JOUR

T1 - Chromosomal assignment and genomic structure of Il15

AU - Anderson, Dirk M.

AU - Johnson, Lisabeth

AU - Glaccum, Moira B.

AU - Copeland, Neal G.

AU - Gilbert, Debra J.

AU - Jenkins, Nancy A.

AU - Valentine, Virginia

AU - Kirstein, Mark N.

AU - Shapiro, David N.

AU - Morris, Stephan W.

AU - Grabstein, Kenneth

AU - Cosman, David

N1 - Funding Information: The authors thank Debbie Barnhart for excellent technical assistance and Anthony Namen for the murine fetal liver epithelial cell line. This research was supported, in part, by the National Cancer Institute (NCI), DHHS, under Contract NOl-CO-74101, NC1 Grant CA01702 (S.W.M.), by Cancer Center CORE Grants CA21765 and CA23099 from the NCI, and by the American Lebanese Syrian Associated Charities (ALSAC).

PY - 1995

Y1 - 1995

N2 - Interleukin-15 (IL-15) is a novel cytokine whose effects on T-cell activation and proliferation are similar to those of interleukin-2 (IL-2), presumably because IL-15 utilizes the β and γ chains of the IL-2 receptor. Murine IL-15 cDNA and genomic clones were isolated and characterized. The murine Il15 gene was found to consist of eight exons spanning at least 34 kb and was localized to the central region of mouse chromosome 8 by interspecific backcross analysis. Intron positions in a partial human IL15 genomic clone were identical with positions of corresponding introns in the murine gene. The human IL15 gene was mapped to human chromosome 4q31 by fluorescence in situ hybridization.

AB - Interleukin-15 (IL-15) is a novel cytokine whose effects on T-cell activation and proliferation are similar to those of interleukin-2 (IL-2), presumably because IL-15 utilizes the β and γ chains of the IL-2 receptor. Murine IL-15 cDNA and genomic clones were isolated and characterized. The murine Il15 gene was found to consist of eight exons spanning at least 34 kb and was localized to the central region of mouse chromosome 8 by interspecific backcross analysis. Intron positions in a partial human IL15 genomic clone were identical with positions of corresponding introns in the murine gene. The human IL15 gene was mapped to human chromosome 4q31 by fluorescence in situ hybridization.

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U2 - 10.1016/0888-7543(95)80013-C

DO - 10.1016/0888-7543(95)80013-C

M3 - Article

C2 - 7759105

AN - SCOPUS:0028969792

SN - 0888-7543

VL - 25

SP - 701

EP - 706

JO - Genomics

JF - Genomics

IS - 3

ER -

Chromosomal assignment and genomic structure of Il15

Abstract

ASJC Scopus subject areas

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