Chitosan/AuNPs Modified Graphene Electrochemical Sensor for Label-Free Human Chorionic Gonadotropin Detection

Sofia Teixeira; Nadia S. Ferreira; Robert Steven Conlan; O. J. Guy; M. Goreti F. Sales

doi:10.1002/elan.201400311

Chitosan/AuNPs Modified Graphene Electrochemical Sensor for Label-Free Human Chorionic Gonadotropin Detection

Sofia Teixeira, Nadia S. Ferreira, Robert Steven Conlan, O. J. Guy, M. Goreti F. Sales

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

A new immunosensor is presented for human chorionic gonadotropin (hCG), made by electrodepositing chitosan/gold-nanoparticles over graphene screen-printed electrode (SPE). The antibody was covalently bound to CS via its Fc-terminal. The assembly was controlled by electrochemical Impedance Spectroscopy (EIS) and followed by Fourier Transformed Infrared (FTIR). The hCG-immunosensor displayed linear response against the logarithm-hCG concentration for 0.1-25ng/mL with limit of detection of 0.016ng/mL. High selectivity was observed in blank urine and successful detection of hCG was also achieved in spiked samples of real urine from pregnant woman. The immunosensor showed good detection capability, simplicity of fabrication, low-cost, high sensitivity and selectivity.

Original language	English (US)
Pages (from-to)	2591-2598
Number of pages	8
Journal	Electroanalysis
Volume	26
Issue number	12
DOIs	https://doi.org/10.1002/elan.201400311
State	Published - Dec 1 2014

Keywords

Antibody
Chitosan
Graphene
Human chorionic gonadotropin
Immunosensor
Screen-printed electrode

ASJC Scopus subject areas

Analytical Chemistry
Electrochemistry

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10.1002/elan.201400311

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title = "Chitosan/AuNPs Modified Graphene Electrochemical Sensor for Label-Free Human Chorionic Gonadotropin Detection",

abstract = "A new immunosensor is presented for human chorionic gonadotropin (hCG), made by electrodepositing chitosan/gold-nanoparticles over graphene screen-printed electrode (SPE). The antibody was covalently bound to CS via its Fc-terminal. The assembly was controlled by electrochemical Impedance Spectroscopy (EIS) and followed by Fourier Transformed Infrared (FTIR). The hCG-immunosensor displayed linear response against the logarithm-hCG concentration for 0.1-25ng/mL with limit of detection of 0.016ng/mL. High selectivity was observed in blank urine and successful detection of hCG was also achieved in spiked samples of real urine from pregnant woman. The immunosensor showed good detection capability, simplicity of fabrication, low-cost, high sensitivity and selectivity.",

keywords = "Antibody, Chitosan, Graphene, Human chorionic gonadotropin, Immunosensor, Screen-printed electrode",

author = "Sofia Teixeira and Ferreira, {Nadia S.} and Conlan, {Robert Steven} and Guy, {O. J.} and Sales, {M. Goreti F.}",

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doi = "10.1002/elan.201400311",

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T1 - Chitosan/AuNPs Modified Graphene Electrochemical Sensor for Label-Free Human Chorionic Gonadotropin Detection

AU - Teixeira, Sofia

AU - Ferreira, Nadia S.

AU - Conlan, Robert Steven

AU - Guy, O. J.

AU - Sales, M. Goreti F.

PY - 2014/12/1

Y1 - 2014/12/1

N2 - A new immunosensor is presented for human chorionic gonadotropin (hCG), made by electrodepositing chitosan/gold-nanoparticles over graphene screen-printed electrode (SPE). The antibody was covalently bound to CS via its Fc-terminal. The assembly was controlled by electrochemical Impedance Spectroscopy (EIS) and followed by Fourier Transformed Infrared (FTIR). The hCG-immunosensor displayed linear response against the logarithm-hCG concentration for 0.1-25ng/mL with limit of detection of 0.016ng/mL. High selectivity was observed in blank urine and successful detection of hCG was also achieved in spiked samples of real urine from pregnant woman. The immunosensor showed good detection capability, simplicity of fabrication, low-cost, high sensitivity and selectivity.

AB - A new immunosensor is presented for human chorionic gonadotropin (hCG), made by electrodepositing chitosan/gold-nanoparticles over graphene screen-printed electrode (SPE). The antibody was covalently bound to CS via its Fc-terminal. The assembly was controlled by electrochemical Impedance Spectroscopy (EIS) and followed by Fourier Transformed Infrared (FTIR). The hCG-immunosensor displayed linear response against the logarithm-hCG concentration for 0.1-25ng/mL with limit of detection of 0.016ng/mL. High selectivity was observed in blank urine and successful detection of hCG was also achieved in spiked samples of real urine from pregnant woman. The immunosensor showed good detection capability, simplicity of fabrication, low-cost, high sensitivity and selectivity.

KW - Antibody

KW - Chitosan

KW - Graphene

KW - Human chorionic gonadotropin

KW - Immunosensor

KW - Screen-printed electrode

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JF - Electroanalysis

SN - 1040-0397

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Chitosan/AuNPs Modified Graphene Electrochemical Sensor for Label-Free Human Chorionic Gonadotropin Detection

Abstract

Keywords

ASJC Scopus subject areas

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