Chitosan/AuNPs Modified Graphene Electrochemical Sensor for Label-Free Human Chorionic Gonadotropin Detection

Sofia Teixeira, Nadia S. Ferreira, Robert Steven Conlan, O. J. Guy, M. Goreti F. Sales

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

A new immunosensor is presented for human chorionic gonadotropin (hCG), made by electrodepositing chitosan/gold-nanoparticles over graphene screen-printed electrode (SPE). The antibody was covalently bound to CS via its Fc-terminal. The assembly was controlled by electrochemical Impedance Spectroscopy (EIS) and followed by Fourier Transformed Infrared (FTIR). The hCG-immunosensor displayed linear response against the logarithm-hCG concentration for 0.1-25ng/mL with limit of detection of 0.016ng/mL. High selectivity was observed in blank urine and successful detection of hCG was also achieved in spiked samples of real urine from pregnant woman. The immunosensor showed good detection capability, simplicity of fabrication, low-cost, high sensitivity and selectivity.

Original languageEnglish (US)
Pages (from-to)2591-2598
Number of pages8
JournalElectroanalysis
Volume26
Issue number12
DOIs
StatePublished - Dec 1 2014

Keywords

  • Antibody
  • Chitosan
  • Graphene
  • Human chorionic gonadotropin
  • Immunosensor
  • Screen-printed electrode

ASJC Scopus subject areas

  • Analytical Chemistry
  • Electrochemistry

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