Chemical carcinogens induce cadmium resistance and activate metallothionein genes in cadmium sensitive S49 mouse cells

Craig A. Macarthur, Rajani Ramabhadran, Andrew K. Godwin, Russell M. Lebovitz, Michael W. Lieberman

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Treatment of cadmium-sensitive (Cds) metallothionein-negative S49 mouse cells with two direct-acting chemical carcinogens (N-ethylnitrosourea or N-acetoxy-2-acetylamino-fluorene) or with u.v. radiation induced a large increase in phenotypically stable cadmium-resistant (Cdr) variants. In contrast, treatment with any of three agents which alkylate proteins (N-ethylmaleimide, iodoacetate, or phenylmethyl-sulfonyl fluoride) was without effect. Similarly, treatment with 2-acetylaminofluorene (a pre-carcinogen) or with 12-O-tetra-decanoylphorbol-13-acetate (a tumor promoter) did not result in an increase in Cdr variants. Initial studies indicate that in many variants the metallothionein-I gene, the metallo-thionein-II gene, or both have been activated. Thus the induction of cadmium resistance in Cds cells is a potentially useful system to explore the activation of quiescent genes by carcinogens.

Original languageEnglish (US)
Pages (from-to)887-892
Number of pages6
JournalCarcinogenesis
Volume6
Issue number6
DOIs
StatePublished - Jun 1985

ASJC Scopus subject areas

  • Cancer Research

Fingerprint Dive into the research topics of 'Chemical carcinogens induce cadmium resistance and activate metallothionein genes in cadmium sensitive S49 mouse cells'. Together they form a unique fingerprint.

Cite this