TY - JOUR
T1 - Chemical and Biological Characterization of Organic Material from Gasoline Exhaust Particles
AU - Alsberg, Tomas
AU - Stenberg, Ulf
AU - Westerholm, Roger
AU - Stranden, Michael
AU - Rannug, Ulf
AU - Sundvall, Annica
AU - Romert, Lennart
AU - Bernson, Vibeke
AU - Pettersson, Bertll
AU - Toftgård, Rune
AU - Franzén, Bo
AU - Jansson, Maria
AU - Gustafsson, Jan-Ake
AU - Egebäck, Karl Erik
AU - Tejle, Gunnar
PY - 1985/1/1
Y1 - 1985/1/1
N2 - Unfractionated gasoline exhaust particle extracts, silica gel fractions, and recombined fractions were analyzed with capillary gas chromatography-mass spectrometry and tested in three biological test systems based on different end points, namely, genotoxicity (Ames Salmonella mutagenicity test), aryl hydrocarbon hydroxylase (AHH) inducibility (2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) receptor affinity test), and cytotoxicity to pulmonary alveolar macrophages (PAM cytotoxicity test). Fraction I contained aliphatic hydrocarbons and showed no activity in the biological tests. Fraction II contained PAH and showed mutagenicity in the presence of a metabolizing system (S9), TCDD-receptor affinity, and PAM cytotoxicity. Fractions III and IV gave high effects in all bioassays. In these fractions polynuclear aromatic ketones are the most abundant species. Fraction V contained the most polar species, including nitrogen-containing compounds, and showed a much weaker activity in the bioassays as compared to fractions III and IV.
AB - Unfractionated gasoline exhaust particle extracts, silica gel fractions, and recombined fractions were analyzed with capillary gas chromatography-mass spectrometry and tested in three biological test systems based on different end points, namely, genotoxicity (Ames Salmonella mutagenicity test), aryl hydrocarbon hydroxylase (AHH) inducibility (2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) receptor affinity test), and cytotoxicity to pulmonary alveolar macrophages (PAM cytotoxicity test). Fraction I contained aliphatic hydrocarbons and showed no activity in the biological tests. Fraction II contained PAH and showed mutagenicity in the presence of a metabolizing system (S9), TCDD-receptor affinity, and PAM cytotoxicity. Fractions III and IV gave high effects in all bioassays. In these fractions polynuclear aromatic ketones are the most abundant species. Fraction V contained the most polar species, including nitrogen-containing compounds, and showed a much weaker activity in the bioassays as compared to fractions III and IV.
UR - http://www.scopus.com/inward/record.url?scp=0021906798&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0021906798&partnerID=8YFLogxK
U2 - 10.1021/es00131a003
DO - 10.1021/es00131a003
M3 - Article
AN - SCOPUS:0021906798
VL - 19
SP - 43
EP - 50
JO - Environmental Science and Technology
JF - Environmental Science and Technology
SN - 0013-936X
IS - 1
ER -