Chemical and Biological Characterization of Organic Material from Gasoline Exhaust Particles

Tomas Alsberg; Ulf Stenberg; Roger Westerholm; Michael Stranden; Ulf Rannug; Annica Sundvall; Lennart Romert; Vibeke Bernson; Bertll Pettersson; Rune Toftgård; Bo Franzén; Maria Jansson; Jan Ake Gustafsson; Karl Erik Egebäck; Gunnar Tejle

doi:10.1021/es00131a003

Chemical and Biological Characterization of Organic Material from Gasoline Exhaust Particles

Tomas Alsberg, Ulf Stenberg, Roger Westerholm, Michael Stranden, Ulf Rannug, Annica Sundvall, Lennart Romert, Vibeke Bernson, Bertll Pettersson, Rune Toftgård, Bo Franzén, Maria Jansson, Jan Ake Gustafsson, Karl Erik Egebäck, Gunnar Tejle

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139 Scopus citations

Abstract

Unfractionated gasoline exhaust particle extracts, silica gel fractions, and recombined fractions were analyzed with capillary gas chromatography-mass spectrometry and tested in three biological test systems based on different end points, namely, genotoxicity (Ames Salmonella mutagenicity test), aryl hydrocarbon hydroxylase (AHH) inducibility (2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) receptor affinity test), and cytotoxicity to pulmonary alveolar macrophages (PAM cytotoxicity test). Fraction I contained aliphatic hydrocarbons and showed no activity in the biological tests. Fraction II contained PAH and showed mutagenicity in the presence of a metabolizing system (S9), TCDD-receptor affinity, and PAM cytotoxicity. Fractions III and IV gave high effects in all bioassays. In these fractions polynuclear aromatic ketones are the most abundant species. Fraction V contained the most polar species, including nitrogen-containing compounds, and showed a much weaker activity in the bioassays as compared to fractions III and IV.

Original language	English (US)
Pages (from-to)	43-50
Number of pages	8
Journal	Environmental Science and Technology
Volume	19
Issue number	1
DOIs	https://doi.org/10.1021/es00131a003
State	Published - Jan 1 1985

ASJC Scopus subject areas

General Chemistry
Environmental Chemistry

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Alsberg, T., Stenberg, U., Westerholm, R., Stranden, M., Rannug, U., Sundvall, A., Romert, L., Bernson, V., Pettersson, B., Toftgård, R., Franzén, B., Jansson, M., Gustafsson, J. A., Egebäck, K. E., & Tejle, G. (1985). Chemical and Biological Characterization of Organic Material from Gasoline Exhaust Particles. Environmental Science and Technology, 19(1), 43-50. https://doi.org/10.1021/es00131a003

Alsberg, T, Stenberg, U, Westerholm, R, Stranden, M, Rannug, U, Sundvall, A, Romert, L, Bernson, V, Pettersson, B, Toftgård, R, Franzén, B, Jansson, M, Gustafsson, JA, Egebäck, KE & Tejle, G 1985, 'Chemical and Biological Characterization of Organic Material from Gasoline Exhaust Particles', Environmental Science and Technology, vol. 19, no. 1, pp. 43-50. https://doi.org/10.1021/es00131a003

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abstract = "Unfractionated gasoline exhaust particle extracts, silica gel fractions, and recombined fractions were analyzed with capillary gas chromatography-mass spectrometry and tested in three biological test systems based on different end points, namely, genotoxicity (Ames Salmonella mutagenicity test), aryl hydrocarbon hydroxylase (AHH) inducibility (2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) receptor affinity test), and cytotoxicity to pulmonary alveolar macrophages (PAM cytotoxicity test). Fraction I contained aliphatic hydrocarbons and showed no activity in the biological tests. Fraction II contained PAH and showed mutagenicity in the presence of a metabolizing system (S9), TCDD-receptor affinity, and PAM cytotoxicity. Fractions III and IV gave high effects in all bioassays. In these fractions polynuclear aromatic ketones are the most abundant species. Fraction V contained the most polar species, including nitrogen-containing compounds, and showed a much weaker activity in the bioassays as compared to fractions III and IV.",

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doi = "10.1021/es00131a003",

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T1 - Chemical and Biological Characterization of Organic Material from Gasoline Exhaust Particles

AU - Alsberg, Tomas

AU - Stenberg, Ulf

AU - Westerholm, Roger

AU - Stranden, Michael

AU - Rannug, Ulf

AU - Sundvall, Annica

AU - Romert, Lennart

AU - Bernson, Vibeke

AU - Pettersson, Bertll

AU - Toftgård, Rune

AU - Franzén, Bo

AU - Jansson, Maria

AU - Gustafsson, Jan Ake

AU - Egebäck, Karl Erik

AU - Tejle, Gunnar

PY - 1985/1/1

Y1 - 1985/1/1

N2 - Unfractionated gasoline exhaust particle extracts, silica gel fractions, and recombined fractions were analyzed with capillary gas chromatography-mass spectrometry and tested in three biological test systems based on different end points, namely, genotoxicity (Ames Salmonella mutagenicity test), aryl hydrocarbon hydroxylase (AHH) inducibility (2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) receptor affinity test), and cytotoxicity to pulmonary alveolar macrophages (PAM cytotoxicity test). Fraction I contained aliphatic hydrocarbons and showed no activity in the biological tests. Fraction II contained PAH and showed mutagenicity in the presence of a metabolizing system (S9), TCDD-receptor affinity, and PAM cytotoxicity. Fractions III and IV gave high effects in all bioassays. In these fractions polynuclear aromatic ketones are the most abundant species. Fraction V contained the most polar species, including nitrogen-containing compounds, and showed a much weaker activity in the bioassays as compared to fractions III and IV.

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Chemical and Biological Characterization of Organic Material from Gasoline Exhaust Particles

Abstract

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