Abstract
A tandem repeat region in the second intron of the K-ras gene has been reported to be a possible regulatory site for transcription [You et al. Proc Natl Acad Sci USA. 1992; 89: 5804-5808; Chen et al. Proc Natl Acad Sci USA. 1994; 91: 1589-1593; Chen et al. Carcinogenesis. 1994; 15: 2031-2035]. In this study, a second protein-binding site was identified and characterized. It lies downstream (nucleotides 463 to 509) of the tandem repeat region. A T→C base variation at nucleotide 494 was found in all KS strains (which have K-ras alleles identical to those of the susceptible A/J strain) and all K i strains (which have K-ras alleles identical to those of the intermediate CBA/J strain). DNase I footprint analysis indicated a protein binding site within the downstream repeated region in the second intron of the K-ras gene. Gel mobility-shift studies showed differential protein-binding patterns between the Kr strains (which have K-ras alleles identical to those of the resistant C3H/HeJ strain) and the Ks or Ki strains. Southwestern blot analysis of DNA-protein complexes indicated that the 2 repeated regions might bind the same regulatory complex.
Original language | English (US) |
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Pages (from-to) | 179-192 |
Number of pages | 14 |
Journal | Experimental Lung Research |
Volume | 31 |
Issue number | 2 |
DOIs | |
State | Published - Mar 2005 |
Keywords
- K-ras
- Protein binding sites
- Second intron
ASJC Scopus subject areas
- Molecular Biology
- Pulmonary and Respiratory Medicine
- Clinical Biochemistry