Characterization of the subcellular localization of herpes simplex virus type 1 proteins in living cells

Junji Xing; Shuai Wang; You Li; Hong Guo; Lei Zhao; Weiwei Pan; Fusen Lin; Huifang Zhu; Lei Wang; Meili Li; Lin Wang; Chunfu Zheng

doi:10.1007/s00430-010-0175-9

Characterization of the subcellular localization of herpes simplex virus type 1 proteins in living cells

Junji Xing, Shuai Wang, You Li, Hong Guo, Lei Zhao, Weiwei Pan, Fusen Lin, Huifang Zhu, Lei Wang, Meili Li, Lin Wang, Chunfu Zheng

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

In this study, we presented the construction of a library of expression clones for the herpes simplex virus type 1 (HSV-1) proteome and subcellular localization map of HSV-1 proteins in living cells using yellow fluorescent protein (YFP) fusion proteins. As a result, 21 proteins showed cytoplasmic or subcytoplasmic localization, 16 proteins showed nuclear or subnuclear localization, and others were present both in the nucleus and cytoplasm. Interestingly, most capsid proteins showed enriched or exclusive localization in the nucleus, and most of the envelope proteins showed cytoplasmic localization, suggesting that subcellular localization of the proteins correlated with their functions during virus replication. These results present a subcellular localization map of HSV-1 proteins in living cells, which provide useful information to further characterize the functions of these proteins.

Original language	English (US)
Pages (from-to)	61-68
Number of pages	8
Journal	Medical Microbiology and Immunology
Volume	200
Issue number	1
DOIs	https://doi.org/10.1007/s00430-010-0175-9
State	Published - Feb 2011

Keywords

Herpes simplex virus type 1 proteins
Living cell microscopy
Subcellular localization

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Microbiology (medical)

Divisions

Abdominal Transplant

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10.1007/s00430-010-0175-9

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title = "Characterization of the subcellular localization of herpes simplex virus type 1 proteins in living cells",

abstract = "In this study, we presented the construction of a library of expression clones for the herpes simplex virus type 1 (HSV-1) proteome and subcellular localization map of HSV-1 proteins in living cells using yellow fluorescent protein (YFP) fusion proteins. As a result, 21 proteins showed cytoplasmic or subcytoplasmic localization, 16 proteins showed nuclear or subnuclear localization, and others were present both in the nucleus and cytoplasm. Interestingly, most capsid proteins showed enriched or exclusive localization in the nucleus, and most of the envelope proteins showed cytoplasmic localization, suggesting that subcellular localization of the proteins correlated with their functions during virus replication. These results present a subcellular localization map of HSV-1 proteins in living cells, which provide useful information to further characterize the functions of these proteins.",

keywords = "Herpes simplex virus type 1 proteins, Living cell microscopy, Subcellular localization",

author = "Junji Xing and Shuai Wang and You Li and Hong Guo and Lei Zhao and Weiwei Pan and Fusen Lin and Huifang Zhu and Lei Wang and Meili Li and Lin Wang and Chunfu Zheng",

note = "Funding Information: Acknowledgments This study was supported by the Major State Basic Research Development Program of China (2010CB530105 and 2011CB504802); the National Natural Science Foundation of China (30900059, 30870120 and 81000736); the Start-up Fund of the Hundred Talents Program of the Chinese Academy of Sciences (20071010-141). Copyright: Copyright 2011 Elsevier B.V., All rights reserved.",

year = "2011",

month = feb,

doi = "10.1007/s00430-010-0175-9",

language = "English (US)",

volume = "200",

pages = "61--68",

journal = "Medical Microbiology and Immunology",

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T1 - Characterization of the subcellular localization of herpes simplex virus type 1 proteins in living cells

AU - Xing, Junji

AU - Wang, Shuai

AU - Li, You

AU - Guo, Hong

AU - Zhao, Lei

AU - Pan, Weiwei

AU - Lin, Fusen

AU - Zhu, Huifang

AU - Wang, Lei

AU - Li, Meili

AU - Wang, Lin

AU - Zheng, Chunfu

N1 - Funding Information: Acknowledgments This study was supported by the Major State Basic Research Development Program of China (2010CB530105 and 2011CB504802); the National Natural Science Foundation of China (30900059, 30870120 and 81000736); the Start-up Fund of the Hundred Talents Program of the Chinese Academy of Sciences (20071010-141). Copyright: Copyright 2011 Elsevier B.V., All rights reserved.

PY - 2011/2

Y1 - 2011/2

N2 - In this study, we presented the construction of a library of expression clones for the herpes simplex virus type 1 (HSV-1) proteome and subcellular localization map of HSV-1 proteins in living cells using yellow fluorescent protein (YFP) fusion proteins. As a result, 21 proteins showed cytoplasmic or subcytoplasmic localization, 16 proteins showed nuclear or subnuclear localization, and others were present both in the nucleus and cytoplasm. Interestingly, most capsid proteins showed enriched or exclusive localization in the nucleus, and most of the envelope proteins showed cytoplasmic localization, suggesting that subcellular localization of the proteins correlated with their functions during virus replication. These results present a subcellular localization map of HSV-1 proteins in living cells, which provide useful information to further characterize the functions of these proteins.

AB - In this study, we presented the construction of a library of expression clones for the herpes simplex virus type 1 (HSV-1) proteome and subcellular localization map of HSV-1 proteins in living cells using yellow fluorescent protein (YFP) fusion proteins. As a result, 21 proteins showed cytoplasmic or subcytoplasmic localization, 16 proteins showed nuclear or subnuclear localization, and others were present both in the nucleus and cytoplasm. Interestingly, most capsid proteins showed enriched or exclusive localization in the nucleus, and most of the envelope proteins showed cytoplasmic localization, suggesting that subcellular localization of the proteins correlated with their functions during virus replication. These results present a subcellular localization map of HSV-1 proteins in living cells, which provide useful information to further characterize the functions of these proteins.

KW - Herpes simplex virus type 1 proteins

KW - Living cell microscopy

KW - Subcellular localization

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Characterization of the subcellular localization of herpes simplex virus type 1 proteins in living cells

Abstract

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