Characterization of the opposite-strand genes from the mouse bidirectionally transcribed HTF9 locus

Alessandro Bressan; Maria Patrizia Somma; Joe Lewis; Carlo Santolamazza; Neal G. Copeland; Debra J. Gilbert; Nancy A. Jenkins; Patrizia Lavia

doi:10.1016/0378-1119(91)90274-F

Characterization of the opposite-strand genes from the mouse bidirectionally transcribed HTF9 locus

Alessandro Bressan, Maria Patrizia Somma, Joe Lewis, Carlo Santolamazza, Neal G. Copeland, Debra J. Gilbert, Nancy A. Jenkins, Patrizia Lavia

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

The mouse HTF9 locus contains two genes that are bidirectionally transcribed with opposite polarity from a shared CpG-rich island. Both genes were previously shown to be expressed in a housekeeping fashion in mouse. We have now determined the molecular organization of the genes over 12 kb surrounding the island. In addition, we show that the HTF9 locus resides in the proximal region of mouse chromosome 16. We have sequenced the cDNAs corresponding to both divergent transcripts. Both genes appear to code for novel proteins that are structurally unrelated to each other. Finally, we show that both genes are highly conserved and efficiently expressed in human cells.

Original language	English (US)
Pages (from-to)	201-209
Number of pages	9
Journal	Gene
Volume	103
Issue number	2
DOIs	https://doi.org/10.1016/0378-1119(91)90274-F
State	Published - Jul 22 1991

Keywords

bidirectional transcription
CpG island
gene molecular organization
housekeeping expression
interspecific backcross mapping
nucleotide sequence

ASJC Scopus subject areas

Genetics

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10.1016/0378-1119(91)90274-F

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title = "Characterization of the opposite-strand genes from the mouse bidirectionally transcribed HTF9 locus",

abstract = "The mouse HTF9 locus contains two genes that are bidirectionally transcribed with opposite polarity from a shared CpG-rich island. Both genes were previously shown to be expressed in a housekeeping fashion in mouse. We have now determined the molecular organization of the genes over 12 kb surrounding the island. In addition, we show that the HTF9 locus resides in the proximal region of mouse chromosome 16. We have sequenced the cDNAs corresponding to both divergent transcripts. Both genes appear to code for novel proteins that are structurally unrelated to each other. Finally, we show that both genes are highly conserved and efficiently expressed in human cells.",

keywords = "bidirectional transcription, CpG island, gene molecular organization, housekeeping expression, interspecific backcross mapping, nucleotide sequence",

author = "Alessandro Bressan and Somma, {Maria Patrizia} and Joe Lewis and Carlo Santolamazza and Copeland, {Neal G.} and Gilbert, {Debra J.} and Jenkins, {Nancy A.} and Patrizia Lavia",

note = "Funding Information: We thank Paola Lucarelli for the gift of human Southern filters, Roger Greeves for probes and D.A. Swing, C.M. Silan and B. Cho for excellent technicaals sistance. This work was supportedb y Consiglio Nazionale delle Ricerche, by Progetto Finalizzato Ingegneria Genetica and by Fondazione Cenci-Bolognetti (PL) and by the National Cancer Institute, DHSS, under contract NOl-CO-74101 with ABL. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1991",

month = jul,

day = "22",

doi = "10.1016/0378-1119(91)90274-F",

language = "English (US)",

volume = "103",

pages = "201--209",

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T1 - Characterization of the opposite-strand genes from the mouse bidirectionally transcribed HTF9 locus

AU - Bressan, Alessandro

AU - Somma, Maria Patrizia

AU - Lewis, Joe

AU - Santolamazza, Carlo

AU - Copeland, Neal G.

AU - Gilbert, Debra J.

AU - Jenkins, Nancy A.

AU - Lavia, Patrizia

N1 - Funding Information: We thank Paola Lucarelli for the gift of human Southern filters, Roger Greeves for probes and D.A. Swing, C.M. Silan and B. Cho for excellent technicaals sistance. This work was supportedb y Consiglio Nazionale delle Ricerche, by Progetto Finalizzato Ingegneria Genetica and by Fondazione Cenci-Bolognetti (PL) and by the National Cancer Institute, DHSS, under contract NOl-CO-74101 with ABL. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1991/7/22

Y1 - 1991/7/22

N2 - The mouse HTF9 locus contains two genes that are bidirectionally transcribed with opposite polarity from a shared CpG-rich island. Both genes were previously shown to be expressed in a housekeeping fashion in mouse. We have now determined the molecular organization of the genes over 12 kb surrounding the island. In addition, we show that the HTF9 locus resides in the proximal region of mouse chromosome 16. We have sequenced the cDNAs corresponding to both divergent transcripts. Both genes appear to code for novel proteins that are structurally unrelated to each other. Finally, we show that both genes are highly conserved and efficiently expressed in human cells.

AB - The mouse HTF9 locus contains two genes that are bidirectionally transcribed with opposite polarity from a shared CpG-rich island. Both genes were previously shown to be expressed in a housekeeping fashion in mouse. We have now determined the molecular organization of the genes over 12 kb surrounding the island. In addition, we show that the HTF9 locus resides in the proximal region of mouse chromosome 16. We have sequenced the cDNAs corresponding to both divergent transcripts. Both genes appear to code for novel proteins that are structurally unrelated to each other. Finally, we show that both genes are highly conserved and efficiently expressed in human cells.

KW - bidirectional transcription

KW - CpG island

KW - gene molecular organization

KW - housekeeping expression

KW - interspecific backcross mapping

KW - nucleotide sequence

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DO - 10.1016/0378-1119(91)90274-F

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AN - SCOPUS:0025819957

SN - 0378-1119

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EP - 209

JO - Gene

JF - Gene

IS - 2

ER -

Characterization of the opposite-strand genes from the mouse bidirectionally transcribed HTF9 locus

Abstract

Keywords

ASJC Scopus subject areas

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