TY - JOUR
T1 - Characterization of the murine thrombospondin gene
AU - Lawler, Jack
AU - Duquette, Mark
AU - Ferro, Paula
AU - Copeland, Neal G.
AU - Gilbert, Debra J.
AU - Jenkins, Nancy A.
N1 - Funding Information:
We thank D. A. Swing and B. Chor for excellent technical assistance. Dr. Doug Gray, Dr. Rudolf Jaenisch, Dr. Laurie Jackson-Grusby, and Dr. Philip Leder kindly provided genomic libraries. We thank Amy Williams and Dr. Tucker Collins for synthesizing the oligonucleotide. We also thank Dr. Temple Smith, Kathleen Klose, and Susan Russo for help with the computer analysis. The manuscript was typed by Alice Callahan and edited by Sami Lawler. This research was supported, in part, by the National Cancer Institute, DHHS, under Contract NOl-CO-74101 with A.B.L. and by National Institutes of Health Grants HL-28749 and HL-42443 from the National Heart, Lung and Blood Institute.
PY - 1991/11
Y1 - 1991/11
N2 - Thrombospondin is an adhesive glycoprotein that supports cell attachment, spreading, and migration. The murine thrombospondin gene is approximately 18 kb in length and includes 22 exons. Interspecific backcross analysis using progeny derived from matings of ( C57BL 6J × Mus spretus)F1 × C57BL 6J mice indicates that the thrombospondin gene is tightly linked to the Fshb, Actcl, Ltk, and B2M loci on murine chromosome 2. The sequence of the murine gene is very similar to that of the human gene in (1) regions of the promoter, (2) the coding region, and (3) the 3′-untranslated region. The predicted amino acid sequence of the mature murine thrombospondin subunit is 95.1% identical to that of the human. The sequences of these two species are most similar at the regions containing the type 1, 2, and 3 repeats as well as the COOH-terminal globular domain. The thrombospondin promoter is similar to the 5′ flanking region of some housekeeping and growth control genes in that it contains multiple GC-rich regions and lacks a CAAT box. The presence of various consensus sequences suggests that thrombospondin gene expression is regulated by cAMP, cytokines, and steroid hormones.
AB - Thrombospondin is an adhesive glycoprotein that supports cell attachment, spreading, and migration. The murine thrombospondin gene is approximately 18 kb in length and includes 22 exons. Interspecific backcross analysis using progeny derived from matings of ( C57BL 6J × Mus spretus)F1 × C57BL 6J mice indicates that the thrombospondin gene is tightly linked to the Fshb, Actcl, Ltk, and B2M loci on murine chromosome 2. The sequence of the murine gene is very similar to that of the human gene in (1) regions of the promoter, (2) the coding region, and (3) the 3′-untranslated region. The predicted amino acid sequence of the mature murine thrombospondin subunit is 95.1% identical to that of the human. The sequences of these two species are most similar at the regions containing the type 1, 2, and 3 repeats as well as the COOH-terminal globular domain. The thrombospondin promoter is similar to the 5′ flanking region of some housekeeping and growth control genes in that it contains multiple GC-rich regions and lacks a CAAT box. The presence of various consensus sequences suggests that thrombospondin gene expression is regulated by cAMP, cytokines, and steroid hormones.
UR - http://www.scopus.com/inward/record.url?scp=0025950940&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025950940&partnerID=8YFLogxK
U2 - 10.1016/0888-7543(91)90066-N
DO - 10.1016/0888-7543(91)90066-N
M3 - Article
C2 - 1774063
AN - SCOPUS:0025950940
SN - 0888-7543
VL - 11
SP - 587
EP - 600
JO - Genomics
JF - Genomics
IS - 3
ER -