Characterization of the mouse JAB1 cDNA and protein

Mangkey A. Bounpheng; Irena N. Melnikova; Sherry G. Dodds; Heping Chen; Neal G. Copeland; Debra J. Gilbert; Nancy A. Jenkins; Barbara A. Christy

doi:10.1016/S0378-1119(99)00525-9

Characterization of the mouse JAB1 cDNA and protein

Mangkey A. Bounpheng, Irena N. Melnikova, Sherry G. Dodds, Heping Chen, Neal G. Copeland, Debra J. Gilbert, Nancy A. Jenkins, Barbara A. Christy

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

JAB1 was originally described as a transcriptional coactivator of c-Jun and Jun D. Recent data suggests that JAB1 is a component of a large protein complex, the JAB1 signalosome in mammals and the COP9 complex in plants. The JAB1 signalosome is implicated in the phosphorylation of selected transcription factors, while the COP9 complex is involved in repression of photomorphogenesis in Arabidopsis. In this study, we describe the partial characterization of mouse JAB1 (mJAB1). The murine JAB1 protein is encoded by a gene located on mouse chromosome 1. mJAB1 mRNA is abundantly expressed in a variety of adult tissues as well as in mouse embryos. The JAB1 protein was readily detectable in many cell types and localized to both the nucleus and cytoplasm. Endogenous JAB1 protein is relatively stable and its degradation is not perturbed by blocking 26S proteasome activity, suggesting that this protein is not degraded by the ubiquitin-mediated proteolytic pathway. (C) 2000 Elsevier Science B.V. All rights reserved.

Original language	English (US)
Pages (from-to)	41-50
Number of pages	10
Journal	Gene
Volume	242
Issue number	1-2
DOIs	https://doi.org/10.1016/S0378-1119(99)00525-9
State	Published - Jan 25 2000

Keywords

Chromosomal location
mRNA
Proteasome
Protein expression
Subcellular location
Ubiquitin

ASJC Scopus subject areas

Genetics

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10.1016/S0378-1119(99)00525-9

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title = "Characterization of the mouse JAB1 cDNA and protein",

abstract = "JAB1 was originally described as a transcriptional coactivator of c-Jun and Jun D. Recent data suggests that JAB1 is a component of a large protein complex, the JAB1 signalosome in mammals and the COP9 complex in plants. The JAB1 signalosome is implicated in the phosphorylation of selected transcription factors, while the COP9 complex is involved in repression of photomorphogenesis in Arabidopsis. In this study, we describe the partial characterization of mouse JAB1 (mJAB1). The murine JAB1 protein is encoded by a gene located on mouse chromosome 1. mJAB1 mRNA is abundantly expressed in a variety of adult tissues as well as in mouse embryos. The JAB1 protein was readily detectable in many cell types and localized to both the nucleus and cytoplasm. Endogenous JAB1 protein is relatively stable and its degradation is not perturbed by blocking 26S proteasome activity, suggesting that this protein is not degraded by the ubiquitin-mediated proteolytic pathway. (C) 2000 Elsevier Science B.V. All rights reserved.",

keywords = "Chromosomal location, mRNA, Proteasome, Protein expression, Subcellular location, Ubiquitin",

author = "Bounpheng, {Mangkey A.} and Melnikova, {Irena N.} and Dodds, {Sherry G.} and Heping Chen and Copeland, {Neal G.} and Gilbert, {Debra J.} and Jenkins, {Nancy A.} and Christy, {Barbara A.}",

note = "Funding Information: We thank Hidde Ploegh (Harvard Medical School) for generous distribution of the proteasome inhibitor Z-L 3 VS, Harvey Ozer (University of Medicine and Dentistry, New Jersey Medical School, Newark, NJ) for the ts20tg cell line, Maria Gaczynska and Pawel Osmulski (University of Texas Health Science Center, San Antonio, TX) for many helpful discussions. We thank Deborah B. Householder for excellent technical assistance. This project was supported by Grant #R29HD29850 from the National Institutes of Health, Grant #1-FY96-0126 from the March of Dimes Birth Defects Foundation (to BC) and the National Cancer Institute, DHHS, under contract with ABL (to NJ and NC).",

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doi = "10.1016/S0378-1119(99)00525-9",

language = "English (US)",

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T1 - Characterization of the mouse JAB1 cDNA and protein

AU - Bounpheng, Mangkey A.

AU - Melnikova, Irena N.

AU - Dodds, Sherry G.

AU - Chen, Heping

AU - Copeland, Neal G.

AU - Gilbert, Debra J.

AU - Jenkins, Nancy A.

AU - Christy, Barbara A.

N1 - Funding Information: We thank Hidde Ploegh (Harvard Medical School) for generous distribution of the proteasome inhibitor Z-L 3 VS, Harvey Ozer (University of Medicine and Dentistry, New Jersey Medical School, Newark, NJ) for the ts20tg cell line, Maria Gaczynska and Pawel Osmulski (University of Texas Health Science Center, San Antonio, TX) for many helpful discussions. We thank Deborah B. Householder for excellent technical assistance. This project was supported by Grant #R29HD29850 from the National Institutes of Health, Grant #1-FY96-0126 from the March of Dimes Birth Defects Foundation (to BC) and the National Cancer Institute, DHHS, under contract with ABL (to NJ and NC).

PY - 2000/1/25

Y1 - 2000/1/25

N2 - JAB1 was originally described as a transcriptional coactivator of c-Jun and Jun D. Recent data suggests that JAB1 is a component of a large protein complex, the JAB1 signalosome in mammals and the COP9 complex in plants. The JAB1 signalosome is implicated in the phosphorylation of selected transcription factors, while the COP9 complex is involved in repression of photomorphogenesis in Arabidopsis. In this study, we describe the partial characterization of mouse JAB1 (mJAB1). The murine JAB1 protein is encoded by a gene located on mouse chromosome 1. mJAB1 mRNA is abundantly expressed in a variety of adult tissues as well as in mouse embryos. The JAB1 protein was readily detectable in many cell types and localized to both the nucleus and cytoplasm. Endogenous JAB1 protein is relatively stable and its degradation is not perturbed by blocking 26S proteasome activity, suggesting that this protein is not degraded by the ubiquitin-mediated proteolytic pathway. (C) 2000 Elsevier Science B.V. All rights reserved.

AB - JAB1 was originally described as a transcriptional coactivator of c-Jun and Jun D. Recent data suggests that JAB1 is a component of a large protein complex, the JAB1 signalosome in mammals and the COP9 complex in plants. The JAB1 signalosome is implicated in the phosphorylation of selected transcription factors, while the COP9 complex is involved in repression of photomorphogenesis in Arabidopsis. In this study, we describe the partial characterization of mouse JAB1 (mJAB1). The murine JAB1 protein is encoded by a gene located on mouse chromosome 1. mJAB1 mRNA is abundantly expressed in a variety of adult tissues as well as in mouse embryos. The JAB1 protein was readily detectable in many cell types and localized to both the nucleus and cytoplasm. Endogenous JAB1 protein is relatively stable and its degradation is not perturbed by blocking 26S proteasome activity, suggesting that this protein is not degraded by the ubiquitin-mediated proteolytic pathway. (C) 2000 Elsevier Science B.V. All rights reserved.

KW - Chromosomal location

KW - mRNA

KW - Proteasome

KW - Protein expression

KW - Subcellular location

KW - Ubiquitin

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SP - 41

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JO - Gene

JF - Gene

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ER -

Characterization of the mouse JAB1 cDNA and protein

Abstract

Keywords

ASJC Scopus subject areas

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