TY - JOUR
T1 - Characterization of the human pleiotrophin gene
T2 - Promoter region and chromosomal localization
AU - Li, Yue Sheng
AU - Hoffman, Ruth M.
AU - Le Beau, Michelle M.
AU - Espinosa, Rafael
AU - Jenkins, Nancy A.
AU - Gilbert, Debra J.
AU - Copeland, Neal G.
AU - Deuel, Thomas F.
PY - 1992/12/25
Y1 - 1992/12/25
N2 - The protein (PTN) encoded by the pleiotrophin (PTN) gene belongs to a recently described family of heparin-binding cytokines whose expression is temporally and spatially regulated during development. We have now isolated genomic clones of the human PTW gene, characterized its promoter region, determined its transcription initiation site(s), and established functional activity of the PTN promoter. A fragment -550/+191 that contains a CAAT box, no apparent TATA box, and four consensus sites for the binding of MyoD is sufficient to provide optimal promoter activity. A serum response element is found at -559 to -568. We also have identified the human PTN gene on chromosome 7, band q33 and the mouse Pin gene on chromosome 6, respectively. The data thus identify and characterize the 5′ end of the PTN gene and its promoter region, suggest potential regions that may contribute to the regulation of its transcriptional activity, and localize the PTN gene in human and mouse chromosomes.
AB - The protein (PTN) encoded by the pleiotrophin (PTN) gene belongs to a recently described family of heparin-binding cytokines whose expression is temporally and spatially regulated during development. We have now isolated genomic clones of the human PTW gene, characterized its promoter region, determined its transcription initiation site(s), and established functional activity of the PTN promoter. A fragment -550/+191 that contains a CAAT box, no apparent TATA box, and four consensus sites for the binding of MyoD is sufficient to provide optimal promoter activity. A serum response element is found at -559 to -568. We also have identified the human PTN gene on chromosome 7, band q33 and the mouse Pin gene on chromosome 6, respectively. The data thus identify and characterize the 5′ end of the PTN gene and its promoter region, suggest potential regions that may contribute to the regulation of its transcriptional activity, and localize the PTN gene in human and mouse chromosomes.
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M3 - Article
C2 - 1464612
AN - SCOPUS:0027101955
SN - 0021-9258
VL - 267
SP - 26011
EP - 26016
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 36
ER -