Characterization of the genomic structure, chromosomal location, promoter, and developmental expression of the α-globin transcription factor CP2

Steven L. Swendeman, Charles Spielholz, Nancy A. Jenkins, Debra J. Gilbert, Neal G. Copeland, Michael Sheffery

Research output: Contribution to journalArticle

40 Scopus citations

Abstract

We recently cloned murine and human cDNAs that encode CP2, a cellular transcription factor that interacts with the α-globin promoter as well as with additional cellular and viral promoter elements. We have now characterized the genomic structure, chromosome location, promoter, and expression pattern of the factor. Genes for the murine and human mRNAs contained 16 and 15 exons, respectively. Both genes spanned approximately 30 kilobases of chromosomal DNA, and among coding exons, all exon/intron boundaries were conserved. The human gene for CP2 was found to reside on chromosome 12 while the murine gene mapped to the distal end of chromosome 15, near Gdc-1, Wnt-1, and Rarg, a region syntenic with human chromosome 12. The murine and human promoters initiated mRNAs at multiple start sites in a conserved region that spanned more than 450 nucleotides. Lastly, a study of the pattern of CP2 gene expression showed that the factor was expressed in all adult and fetal murine tissues examined from at least day 9.5 of development.

Original languageEnglish (US)
Pages (from-to)11663-11671
Number of pages9
JournalJournal of Biological Chemistry
Volume269
Issue number15
StatePublished - Apr 15 1994

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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