TY - JOUR
T1 - Characterization of the β-adrenergic receptor and adenylate cyclase in skeletal muscle plasma membranes
AU - Grefrath, Stuart P.
AU - Smith, P. Blaise
AU - Appel, Stanley H.
PY - 1978/1/1
Y1 - 1978/1/1
N2 - Subcellular fractions of rat skeletal muscle enriched in plasma membrane (sarcolemma) were used to characterize properties of the β-adrenergic receptor and adenylate cyclase. The equilibrium binding of (-)-[3H]dihydroalprenolol to the sarcolemmal membrane was characterized by a high affinity, K=3.6×109 liters/mol and 1.2×10-9 mol/g of membrane protein of homogenous noninteracting sites. The binding was reversible, saturable, and stereospecific. Displacement of labeled dihydroalprenolol by adrenergic ligands revealed an order of potency corresponding to the β2-type response: (-)-isoproterenol>(-)-epinephrine >norepinephrine. The (+)-stereoisomers and the α-adrenergic antagonist phentolamine showed little ability to compete with (-)-[3H]dihydroalprenolol for occupancy of the binding sites. In the membrane fractions, a substantial basal activity of adenylate cyclase activity was demonstrated (35-50 pmol of cyclic AMP/mg/5 min). This activity was stimulated 40- to 50-fold by sodium fluoride, 7-to 8-fold by isoproterenol, and 5- to 6-fold by guanylyl 5′-imidodiphosphate [Gpp(NH)p]. Maximum activation depended upon optimal Mg2+ ion concentration (10 mm). In the presence of 0.1 mm Gpp (NH)p., the isoproterenol activation of adenylate cyclase was increased twofold. In addition, the concentration of hormone required for half-maximal stimulation of enzyme activity was shifted from 2.2×10-7m in the absence of Gpp(NH)p to 2.5×10-8 m in the presence of Gpp(NH)p. Gpp(NH)p also stimulated epinephrine and norepinephrine activation without altering the β2-type potency series for the enzyme. Gpp(NH)p had no effect on either the number or the affinity of (-)-[3H]dihydroalprenolol binding sites, but it did increase the dissociation constant for isoproterenol by approximately fivefold.
AB - Subcellular fractions of rat skeletal muscle enriched in plasma membrane (sarcolemma) were used to characterize properties of the β-adrenergic receptor and adenylate cyclase. The equilibrium binding of (-)-[3H]dihydroalprenolol to the sarcolemmal membrane was characterized by a high affinity, K=3.6×109 liters/mol and 1.2×10-9 mol/g of membrane protein of homogenous noninteracting sites. The binding was reversible, saturable, and stereospecific. Displacement of labeled dihydroalprenolol by adrenergic ligands revealed an order of potency corresponding to the β2-type response: (-)-isoproterenol>(-)-epinephrine >norepinephrine. The (+)-stereoisomers and the α-adrenergic antagonist phentolamine showed little ability to compete with (-)-[3H]dihydroalprenolol for occupancy of the binding sites. In the membrane fractions, a substantial basal activity of adenylate cyclase activity was demonstrated (35-50 pmol of cyclic AMP/mg/5 min). This activity was stimulated 40- to 50-fold by sodium fluoride, 7-to 8-fold by isoproterenol, and 5- to 6-fold by guanylyl 5′-imidodiphosphate [Gpp(NH)p]. Maximum activation depended upon optimal Mg2+ ion concentration (10 mm). In the presence of 0.1 mm Gpp (NH)p., the isoproterenol activation of adenylate cyclase was increased twofold. In addition, the concentration of hormone required for half-maximal stimulation of enzyme activity was shifted from 2.2×10-7m in the absence of Gpp(NH)p to 2.5×10-8 m in the presence of Gpp(NH)p. Gpp(NH)p also stimulated epinephrine and norepinephrine activation without altering the β2-type potency series for the enzyme. Gpp(NH)p had no effect on either the number or the affinity of (-)-[3H]dihydroalprenolol binding sites, but it did increase the dissociation constant for isoproterenol by approximately fivefold.
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U2 - 10.1016/S0003-9861(78)80017-4
DO - 10.1016/S0003-9861(78)80017-4
M3 - Article
C2 - 209751
AN - SCOPUS:0018188543
SN - 0003-9861
VL - 188
SP - 328
EP - 337
JO - Archives of Biochemistry and Biophysics
JF - Archives of Biochemistry and Biophysics
IS - 2
ER -