TY - JOUR
T1 - Characterization of TCDD-receptor ligands present in extracts of urban particulate matter
AU - Toftgård, Rune
AU - Franzén, Bo
AU - Gustafsson, Jan Åke
AU - Löfroth, Göran
N1 - Funding Information:
Acknowledgements-This study was supported by a grant from the National Swedish Environment Protection Board through Project: Air Pollution in Urban Areas.
PY - 1985
Y1 - 1985
N2 - Extracts of filter-collected urban airborne particulate matter contain compounds which can competitively inhibit 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) binding to the rat liver TCDD-receptor protein. The concentration of conventional polycyclic aromatic hydrocarbons (PAHs) or chlorinated dioxins and dibenzofurans cannot account for more than 1%-30% of the observed competition for TCDD-binding to the receptor protein. Chemical fractionation of an extract of a well-characterized particulate air sample collected in Washington, DC showed the highest activity residing in the hexane/benzene fraction, although significant levels of activity were found also in other fractions. Analysis of extracts of gasoline exhaust particulate matter and fractions thereof gave similar results. A series of pure PAHs and nitro-and chloroderivatives of PAHs was tested for competition with TCDD for receptor binding. Among the most potent compounds with EC50-values similar to tetrachlorodibenzofuran were 1- and 3-nitrobenzo(a)-pyrene. Other highly active compounds included dibenzo(a,h)anthracene, benzo(j)fluoranthene, benzo(k)fluoranthene, picene, indeno(1,2,3-cd)pyrene, 3-nitroperylene, and 3,9-and 3,10-dinitroperylene. the EC50-value for aryl hydrocarbon hydroxylase induction in hepatoma cells and the EC50-value for TCDD-receptor binding were similar for dibenzo(a,h)anthracene and for a PAH-containing fraction of an extract of gasoline exhaust particulates. The presence of active TCDD-receptor ligands in extracts of urban particulate matter and their ability to be taken up by cells and cause biological alterations represent a potential health risk. The identity of these compounds are largely unknown although certain PAHs and possibly some nitroderivatives of PAHs are likely to play a role.
AB - Extracts of filter-collected urban airborne particulate matter contain compounds which can competitively inhibit 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) binding to the rat liver TCDD-receptor protein. The concentration of conventional polycyclic aromatic hydrocarbons (PAHs) or chlorinated dioxins and dibenzofurans cannot account for more than 1%-30% of the observed competition for TCDD-binding to the receptor protein. Chemical fractionation of an extract of a well-characterized particulate air sample collected in Washington, DC showed the highest activity residing in the hexane/benzene fraction, although significant levels of activity were found also in other fractions. Analysis of extracts of gasoline exhaust particulate matter and fractions thereof gave similar results. A series of pure PAHs and nitro-and chloroderivatives of PAHs was tested for competition with TCDD for receptor binding. Among the most potent compounds with EC50-values similar to tetrachlorodibenzofuran were 1- and 3-nitrobenzo(a)-pyrene. Other highly active compounds included dibenzo(a,h)anthracene, benzo(j)fluoranthene, benzo(k)fluoranthene, picene, indeno(1,2,3-cd)pyrene, 3-nitroperylene, and 3,9-and 3,10-dinitroperylene. the EC50-value for aryl hydrocarbon hydroxylase induction in hepatoma cells and the EC50-value for TCDD-receptor binding were similar for dibenzo(a,h)anthracene and for a PAH-containing fraction of an extract of gasoline exhaust particulates. The presence of active TCDD-receptor ligands in extracts of urban particulate matter and their ability to be taken up by cells and cause biological alterations represent a potential health risk. The identity of these compounds are largely unknown although certain PAHs and possibly some nitroderivatives of PAHs are likely to play a role.
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U2 - 10.1016/0160-4120(85)90031-5
DO - 10.1016/0160-4120(85)90031-5
M3 - Article
AN - SCOPUS:0022431410
SN - 0160-4120
VL - 11
SP - 369
EP - 374
JO - Environment International
JF - Environment International
IS - 2-4
ER -