Characterization of magnesium requirement of human 5'-tyrosyl DNA phosphodiesterase mediated reaction

Sanjay Adhikari, Soumendra K. Karmahapatra, Tejaswita M. Karve, Sanjona Bandyopadhyay, Jordan Woodrick, Praveen V. Manthena, Eric Glasgow, Stephen Byers, Tapas Saha, Aykut Uren

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Background: Topo-poisons can produce an enzyme-DNA complex linked by a 3'- or 5'-phosphotyrosyl covalent bond. 3'-phosphotyrosyl bonds can be repaired by tyrosyl DNA phosphodiesterase-1 (TDP1), an enzyme known for years, but a complementary human enzyme 5'-tyrosyl DNA phosphodiesterase (hTDP2) that cleaves 5'-phosphotyrosyl bonds has been reported only recently. Although hTDP2 possesses both 3'- and 5'- tyrosyl DNA phosphodiesterase activity, the role of Mg 2+ in its activity was not studied in sufficient details. Results: In this study we showed that purified hTDP2 does not exhibit any 5'-phosphotyrosyl phosphodiesterase activity in the absence of Mg 2+/Mn 2+, and that neither Zn 2+ or nor Ca 2+ can activate hTDP2. Mg 2+ also controls 3'-phosphotyrosyl activity of TDP2. In MCF-7 cell extracts and de-yolked zebrafish embryo extracts, Mg 2+ controlled 5'-phosphotyrosyl activity. This study also showed that there is an optimal Mg 2+ concentration above which it is inhibitory for hTDP2 activity. Conclusion: These results altogether reveal the optimal Mg 2+ requirement in hTDP2 mediated reaction.

Original languageEnglish (US)
Article number134
JournalBMC Research Notes
Volume5
DOIs
StatePublished - 2012

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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