Characterization of macrolide resistance in Gram-positive cocci from Colombian hospitals: a countrywide surveillance

Jinnethe Reyes, Marylin Hidalgo, Lorena Díaz, Sandra Rincón, Jaime Moreno, Natasha Vanegas, Elizabeth Castañeda, César A. Arias

Research output: Contribution to journalArticlepeer-review

42 Scopus citations

Abstract

Objective: The characterization of macrolide resistance in Gram-positive cocci recovered from Colombian hospitals. Methods: The resistance profiles and mechanism of macrolide resistance were investigated in isolates of Streptococcus pneumoniae (1679), Staphylococcus aureus (348), coagulase-negative staphylococci (CoNS) (175), and Enterococcus spp (123). Minimum inhibitory concentrations (MICs) for erythromycin (ERY) and clindamycin (CLI), detection of macrolide resistance genes, phenotypic characterization, and pulsed field gel electrophoresis (PFGE) of macrolide-resistant pneumococci were performed. Results: Resistance to ERY and CLI was 3.3% and 2.3% for S. pneumoniae, 58% and 57% for S. aureus (94% for both compounds in methicillin-resistant Staphylococcus aureus (MRSA)), and 78.6% and 60.7% in methicillin-resistant Staphylococcus epidermidis, respectively. ERY resistance was 62% in Enterococcus faecalis and 82% in Enterococcus faecium. The MLSB-type accounted for 71% of S. pneumoniae and 100% of MRSA. The erm(A) gene was prevalent in MRSA, erm(B) in S. pneumoniae and enterococci, and erm(C) in CoNS isolates. Efflux pump genes (mef(A) genes) were mostly identified in S. pneumoniae (24%). The most common genotype amongst ERY-resistant pneumococci was the Spain6B-2 clone. Conclusions: The prevalence of macrolide resistance is low in Colombian pneumococci and high in MRSA (cMLSB-type).

Original languageEnglish (US)
Pages (from-to)329-336
Number of pages8
JournalInternational Journal of Infectious Diseases
Volume11
Issue number4
DOIs
StatePublished - Jul 2007

Keywords

  • Antimicrobial drug resistance
  • Enterococcus
  • Macrolides
  • Staphylococcus
  • Streptococcus pneumoniae

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

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