Characterization of human thioredoxin-like-1: Potential involvement in the cellular response against glucose deprivation

Alberto Jiménez, Markku Pelto-Huikko, Jan Åke Gustafsson, Antonio Miranda-Vizuete

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

The thioredoxin system, composed of thioredoxin (Trx) and thioredoxin reductase (TrxR), emerges as one of the most important thiol-based systems involved in the maintenance of the cellular redox balance. Thioredoxin-like-1 (TXL-1) is a highly conserved protein comprising an N-terminal Trx domain and a C-terminal domain of unknown function. Here we show that TXL-1 is a substrate for the cytosolic selenoprotein TrxR-1. In situ hybridization experiments demonstrates high expression of Txl-1 mRNA in various areas of central nervous system and also in some reproductive organs. Glucose deprivation, but not hydrogen peroxide treatment, reduced the levels of endogenous TXL-1 protein in HEK-293 cell line. Conversely, overexpression of TXL-1 protects against glucose deprivation-induced cytotoxicity. Taken together, the finding that Txl-1 mRNA is highly expressed in tissues which use glucose as a primary energy source and the modulation of TXL-1 levels upon glucose deprivation indicate that TXL-1 might be involved in the cellular response to sugar starvation stress.

Original languageEnglish (US)
Pages (from-to)960-967
Number of pages8
JournalFEBS Letters
Volume580
Issue number3
DOIs
StatePublished - Feb 6 2006

Keywords

  • Cell death
  • Glucose deprivation
  • Stress
  • Thioredoxin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Fingerprint Dive into the research topics of 'Characterization of human thioredoxin-like-1: Potential involvement in the cellular response against glucose deprivation'. Together they form a unique fingerprint.

Cite this