TY - JOUR
T1 - Characterization of human DSPG3, a small dermatan sulfate proteoglycan
AU - Deere, Michelle
AU - Johnson, Jan
AU - Garza, Sonya
AU - Harrison, Wilbur R.
AU - Yoon, Sung Joo
AU - Elder, Frederick F.B.
AU - Kucherlapati, Raju
AU - Hook, Magnus
AU - Hecht, Jacqueline T.
N1 - Funding Information:
We thank Drs. Yoshi Yamada, William Horton, and Brendon Lee for providing the chondrocyte cDNA libraries, Dr. Stephen P. Daiger for the use of his BIOSMAP kit, Tim Ritty, Yang Wang, and Dr. Jason Hou for extracting RNA from the various tissues, and Deborah Hogue for making the cartilage Northern blot. We also thank Drs. Ralf Krahe, Joe Rodriguez, Paul Au, and Liz Putnam for technical advice. We thank Clontech Laboratories, Inc., for providing the Marathon cDNA Ampli®cation kit and PromoterFinder DNA Walking kit. This research was supported by Shriner's Hospital for Crippled Children Grants 15955 and 15957 to J.T.H. and Grant NCI CA-16672 for the Genetics Computer Group.
PY - 1996/12/15
Y1 - 1996/12/15
N2 - PG-Lb is a small dermatan sulfate proteoglycan that has been previously characterized in chicken. In the developing limb, chick PG-Lb appears to be exclusively expressed in the zone of flattened chondrocytes. We have cloned and sequenced the human homolog to chick PG-Lb from two human chondrocyte cDNA libraries and a human chondrocyte RNA sample. The human homolog has been named DSPG3, as it is the third member of the small dermatan sulfate proteoglycan family to be identified and characterized along with biglycan (PG-I) and decorin (PG-II). DSPG3 maps to chromosome 12q21 and is composed of 1515 nucleotides of cDNA that code for a 322-amino-acid protein. The protein contains three potential glycosaminoglycan attachment sites, two N- glycosylation sites, a polyglutamic acid stretch, and six cysteines. By Northern analysis, we have demonstrated that DSPG3 is expressed in cartilage, as well as ligament and placental tissues.
AB - PG-Lb is a small dermatan sulfate proteoglycan that has been previously characterized in chicken. In the developing limb, chick PG-Lb appears to be exclusively expressed in the zone of flattened chondrocytes. We have cloned and sequenced the human homolog to chick PG-Lb from two human chondrocyte cDNA libraries and a human chondrocyte RNA sample. The human homolog has been named DSPG3, as it is the third member of the small dermatan sulfate proteoglycan family to be identified and characterized along with biglycan (PG-I) and decorin (PG-II). DSPG3 maps to chromosome 12q21 and is composed of 1515 nucleotides of cDNA that code for a 322-amino-acid protein. The protein contains three potential glycosaminoglycan attachment sites, two N- glycosylation sites, a polyglutamic acid stretch, and six cysteines. By Northern analysis, we have demonstrated that DSPG3 is expressed in cartilage, as well as ligament and placental tissues.
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U2 - 10.1006/geno.1996.0643
DO - 10.1006/geno.1996.0643
M3 - Article
C2 - 8975717
AN - SCOPUS:0030589590
SN - 0888-7543
VL - 38
SP - 399
EP - 404
JO - Genomics
JF - Genomics
IS - 3
ER -