The developmental pathways and differentiation relationship of dendritic cell (DC) subsets remain unclear. We report that murine CD11c+MHC II- bone marrow cells, which are immediate DC precursors of CD8α+, CD8α-, and B220+ DC in vivo, can be separated into B220+ and B220- DC precursor subpopulations. Purified B220- DC precursors expand, and generate exclusively mature CD11c+CD11b+B220- DC in vitro and after adoptive transfer. B220+ DC precursors, which resemble plasmacytoid pre-DC, have a lower proliferate potential than B220 - DC precursors and generate both CD11b- B220+ and CD11b+B22- DC populations. Both DC precursor populations can give rise to CD8α+ and CD8α- DC subtypes. Our findings indicate that CD11c+MHC II -B220+ and CD11c+MHC II-B22 - bone marrow cells are distinct DC lineage-restricted precursors.
ASJC Scopus subject areas
- Immunology and Allergy