Characterization of colorectal-cancer-related cDNA clones obtained by subtractive hybridization screening

Jiang Cao, Xinhan Cai, Lei Zheng, Liyi Geng, Zhengzheng Shi, Chia C. Pao, Shu Zheng

    Research output: Contribution to journalArticlepeer-review

    42 Scopus citations

    Abstract

    In an attempt to seek out new factors that are related to colorectal carcinogenesis at the molecular level, subtractive hybridization between cDNA of normal mucosal tissues and mRNA of colorectal carcinoma tissues was performed. Subsequent screenings of the cDNA libraries, constructed from normal mucosal tissues, using the 'subtractive probes' generated a total of 46 clones that were expressed in normal mucosa but were either expressed at a significantly reduced level or not expressed at all in cancer tissues. Partial nucleotide sequences of all of these cDNA clones were determined, and sequence homology analyses were performed with the Genbank database. Of the 46 cDNA samples, 44 contained substantial sequence homologies with 32 immunoglobulin gene fragments, a helix-loop-helix basic phosphoprotein gene, an acidic ribosomal phosphoprotein P2 gene, a BLR1 gene for Burkitt's lymphoma receptor 1 gene, D5S419 DNA segment containing (C-A) repeats, a glucokinase (GCK) gene, a Na +, K +-ATPase α-subunit gene, a histocompatibility system HLA-DR heavy-chain gene, a dystrophic gene, a mucin (MUC2) gene, a μ-glutathione S-transferase gene, a Menkes disease protein gene, and a 40-kDa keratin intermediate filament precursor gene. The remaining two cDNA clones (now registered under GenBank accession numbers U17714 and U20428) showed few (less than 60%) sequence homologies with any known sequences in the GenBank database and, therefore, may represent novel genes whose expression was down-regulated in human colorectal carcinomas. The possible clinical significance of these findings and the involvement of these two genes in the carcinogenesis of colorectal as well as other cancers are being investigated.

    Original languageEnglish (US)
    Pages (from-to)447-451
    Number of pages5
    JournalJournal of Cancer Research and Clinical Oncology
    Volume123
    Issue number8
    DOIs
    StatePublished - 1997

    Keywords

    • Colorectal neoplasm
    • Down-regulation
    • Gene expression
    • Sequence analysis
    • Subtractive nucleic and acid hybridization

    ASJC Scopus subject areas

    • Cancer Research
    • Oncology

    Fingerprint

    Dive into the research topics of 'Characterization of colorectal-cancer-related cDNA clones obtained by subtractive hybridization screening'. Together they form a unique fingerprint.

    Cite this