Characterization of bioimprinted tannase and its kinetic and thermodynamics properties in synthesis of propyl gallate by transesterification in anhydrous medium

Guangjun Nie, Zhiming Zheng, Guohong Gong, Genhai Zhao, Yan Liu, Junying Song, Jun Dai

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Tannase has been extensively applied to synthesize gallic acid esters. Bioimprinting technique can evidently enhance transesterification-catalyzing performance of tannase. In order to promote the practical utilization of the modified tannase, a few enzymatic characteristics of the enzyme and its kinetic and thermodynamics properties in synthesis of propyl gallate by transesterification in anhydrous medium have been studied. The investigations of pH and temperature found that the imprinted tannase holds an optimum activity at pH 5.0 and 40 °C. On the other hand, the bioimprinting technique has a profound enhancing effect on the adapted tannase in substrate affinity and thermostability. The kinetic and thermodynamic analyses showed that the modified tannase has a longer half-time of 1,710 h at 40 °C; the kinetic constants, the activation energy of reversible thermal inactivation, and the activation energy of irreversible thermal inactivation, respectively, are 0.054 mM, 17.35 kJ mol-1, and 85.54 kJ mol-1 with tannic acid as a substrate at 40 °C; the free energy of Gibbs (ΔG) and enthalpy (ΔH) were found to be 97.1 and 82.9 kJ mol-1 separately under the same conditions.

Original languageEnglish (US)
Pages (from-to)2305-2317
Number of pages13
JournalApplied Biochemistry and Biotechnology
Volume167
Issue number8
DOIs
StatePublished - Aug 1 2012

Keywords

  • Bioimprinting
  • Kinetic
  • Tannase
  • Thermodynamics
  • Transesterification

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Molecular Biology

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