Characterization of a YAC and cosmid contig containing markers tightly linked to the myotonic dystrophy locus on chromosome 19

Jessica Buxton; Peggy Shelbourne; June Davies; Clare Jones; M. Benjamin Perryman; Tetsuo Ashizawa; Rachel Butler; David Brook; Duncan Shaw; Pieter de Jong; Alex Markham; Robert Williamson; Keith Johnson

doi:10.1016/0888-7543(92)90120-H

Characterization of a YAC and cosmid contig containing markers tightly linked to the myotonic dystrophy locus on chromosome 19

Jessica Buxton, Peggy Shelbourne, June Davies, Clare Jones, M. Benjamin Perryman, Tetsuo Ashizawa, Rachel Butler, David Brook, Duncan Shaw, Pieter de Jong, Alex Markham, Robert Williamson, Keith Johnson

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Myotonic dystrophy (DM) is caused by a defect in an unknown gene that maps to 19q13.3, flanked by the tightly linked markers ERCC1 on the proximal side and D19S51 on the distal side. We report the isolation and characterization of overlapping YAC and cosmid clones around D19S51 for the construction of a physical map around this locus. The resulting contig contains the markers D19S51 and D19S62 (another new marker tightly linked to the DM locus) and the distal breakpoint of a radiation hybrid cell line used in the physical mapping of the DM region. We have compared the restriction maps of the YACs and cosmids with that of the genome to investigate the fidelity of these clones.

Original language	English (US)
Pages (from-to)	526-531
Number of pages	6
Journal	Genomics
Volume	13
Issue number	3
DOIs	https://doi.org/10.1016/0888-7543(92)90120-H
State	Published - Jul 1992

ASJC Scopus subject areas

Genetics

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Buxton, J., Shelbourne, P., Davies, J., Jones, C., Perryman, M. B., Ashizawa, T., Butler, R., Brook, D., Shaw, D., Jong, P. D., Markham, A., Williamson, R., & Johnson, K. (1992). Characterization of a YAC and cosmid contig containing markers tightly linked to the myotonic dystrophy locus on chromosome 19. Genomics, 13(3), 526-531. https://doi.org/10.1016/0888-7543(92)90120-H

Buxton, J, Shelbourne, P, Davies, J, Jones, C, Perryman, MB, Ashizawa, T, Butler, R, Brook, D, Shaw, D, Jong, PD, Markham, A, Williamson, R & Johnson, K 1992, 'Characterization of a YAC and cosmid contig containing markers tightly linked to the myotonic dystrophy locus on chromosome 19', Genomics, vol. 13, no. 3, pp. 526-531. https://doi.org/10.1016/0888-7543(92)90120-H

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title = "Characterization of a YAC and cosmid contig containing markers tightly linked to the myotonic dystrophy locus on chromosome 19",

abstract = "Myotonic dystrophy (DM) is caused by a defect in an unknown gene that maps to 19q13.3, flanked by the tightly linked markers ERCC1 on the proximal side and D19S51 on the distal side. We report the isolation and characterization of overlapping YAC and cosmid clones around D19S51 for the construction of a physical map around this locus. The resulting contig contains the markers D19S51 and D19S62 (another new marker tightly linked to the DM locus) and the distal breakpoint of a radiation hybrid cell line used in the physical mapping of the DM region. We have compared the restriction maps of the YACs and cosmids with that of the genome to investigate the fidelity of these clones.",

author = "Jessica Buxton and Peggy Shelbourne and June Davies and Clare Jones and Perryman, {M. Benjamin} and Tetsuo Ashizawa and Rachel Butler and David Brook and Duncan Shaw and Jong, {Pieter de} and Alex Markham and Robert Williamson and Keith Johnson",

year = "1992",

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doi = "10.1016/0888-7543(92)90120-H",

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T1 - Characterization of a YAC and cosmid contig containing markers tightly linked to the myotonic dystrophy locus on chromosome 19

AU - Buxton, Jessica

AU - Shelbourne, Peggy

AU - Davies, June

AU - Jones, Clare

AU - Perryman, M. Benjamin

AU - Ashizawa, Tetsuo

AU - Butler, Rachel

AU - Brook, David

AU - Shaw, Duncan

AU - Jong, Pieter de

AU - Markham, Alex

AU - Williamson, Robert

AU - Johnson, Keith

PY - 1992/7

Y1 - 1992/7

N2 - Myotonic dystrophy (DM) is caused by a defect in an unknown gene that maps to 19q13.3, flanked by the tightly linked markers ERCC1 on the proximal side and D19S51 on the distal side. We report the isolation and characterization of overlapping YAC and cosmid clones around D19S51 for the construction of a physical map around this locus. The resulting contig contains the markers D19S51 and D19S62 (another new marker tightly linked to the DM locus) and the distal breakpoint of a radiation hybrid cell line used in the physical mapping of the DM region. We have compared the restriction maps of the YACs and cosmids with that of the genome to investigate the fidelity of these clones.

AB - Myotonic dystrophy (DM) is caused by a defect in an unknown gene that maps to 19q13.3, flanked by the tightly linked markers ERCC1 on the proximal side and D19S51 on the distal side. We report the isolation and characterization of overlapping YAC and cosmid clones around D19S51 for the construction of a physical map around this locus. The resulting contig contains the markers D19S51 and D19S62 (another new marker tightly linked to the DM locus) and the distal breakpoint of a radiation hybrid cell line used in the physical mapping of the DM region. We have compared the restriction maps of the YACs and cosmids with that of the genome to investigate the fidelity of these clones.

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Characterization of a YAC and cosmid contig containing markers tightly linked to the myotonic dystrophy locus on chromosome 19

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