CHARACTERIZATION OF A BOTTLENOSE DOLPHIN (TURSIOPS TRUNCATUS) KIDNEY EPITHELIAL CELL LINE

Michael J. Carvan, MICHAEL SANTOSTEFANO, STEPHEN SAFE, DAVID BUSBEE

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

Abstract: An epithelial cell line, Carvan dolphin kidney (CDK), isolated from a prematurely born female bottlenose dolphin, Tursiops truncatus, exhibited growth characteristics not previously reported for cetacean cells in culture. CDK cells were cytokeratin positive and demonstrated a maximum doubling time of 1.31 days, with plating and colony forming efficiencies approaching 100% for the early population doublings. Despite an unusually efficient colony‐forming ability and rapid growth, these cells were neither transformed nor immortal, displaying normal contact inhibition, anchorage dependence, and the requirement for high concentrations of fetal bovine serum in the growth medium. CDK cells exhibited age‐dependent changes in growth rate, colony‐forming efficiency, and cytoplasmic profile, and showed a finite lifespan of about 50 population doublings and a stable 2N = 44 karyotype which correlates with previously reported cytogenetic analyses. Velocity sedimentation analysis showed that CDK cells contained nuclear aryl hydrocarbon receptor (Ah), indicating their potential to be induced for cytochrome P450. These data suggest that CDK cells may have utility as an in vitro toxicological model for evaluating hydrocarbon contaminant effects on Tursiops truncatus, a protected marine mammal.

Original languageEnglish (US)
Pages (from-to)52-69
Number of pages18
JournalMarine Mammal Science
Volume10
Issue number1
DOIs
StatePublished - Jan 1994

Keywords

  • Ah receptor
  • bottlenose dolphin
  • karyotype
  • kidney epithelial cell
  • tissue culture
  • Tursiops truncatus

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science

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