Characterization, Bioactivity, and Biodistribution of 35 kDa Hyaluronan Fragment

Munkh Amgalan Gantumur, Xiaoxiao Jia, Jessica H. Hui, Christy Barber, Li Wan, Lars R. Furenlid, Diego R. Martin, Mizhou Hui, Zhonglin Liu

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

It has been reported that hyaluronic acid (HA) with a 35 kDa molecular weight (HA35) acts biologically to protect tissue from injury, but its biological properties are not yet fully characterized. This study aimed to evaluate the cellular effects and biodistribution of HA35 compared to HA with a 1600 kDa molecular weight (HA1600). We assessed the effects of HA35 and HA1600 on cell migration, NO and ROS generation, and gene expression in cultured macrophages, microglia, and lymphocytes. HA35 was separately radiolabeled with 99mTc and 125I and administered to C57BL/6J mice for in vivo biodistribution imaging. In vitro studies indicated that HA35 and HA1600 similarly enhanced cell migration through HA receptor binding mechanisms, reduced the generation of NO and ROS, and upregulated gene expression profiles related to cell signaling pathways in immune cells. HA35 showed a more pronounced effect in regulating a broader range of genes in macrophages and microglia than HA1600. Upon intradermal or intravenous administration, radiolabeled HA35 rapidly accumulated in the liver, spleen, and lymph nodes. In conclusion, HA35 not only exhibits effects on cellular bioactivity comparable to those of HA1600 but also exerts biological effects on a broader range of immune cell gene expression. The findings herein offer valuable insights for further research into the therapeutic potential of HA35 in inflammation-mediated tissue injury.

Original languageEnglish (US)
Article number97
JournalLife
Volume14
Issue number1
DOIs
StatePublished - Jan 2024

Keywords

  • cell migration
  • hyaluronan (HA)
  • immune cells
  • inflammatory biomarkers
  • low-molecular-weight HA (LMW-HA) fragment

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Biochemistry, Genetics and Molecular Biology(all)
  • Space and Planetary Science
  • Palaeontology

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