TY - JOUR
T1 - Characterisation of white and yellow eye colour mutant strains of house cricket, Acheta domesticus
AU - Francikowski, Jacek
AU - Krzyżowski, Michał
AU - Kochańska, Barbara
AU - Potrzebska, Marta
AU - Baran, Bartosz
AU - Chajec, Łukasz
AU - Urbisz, Anna
AU - Małota, Karol
AU - Łozowski, Bartosz
AU - Kloc, Malgorzata
AU - Kubiak, Jacek
PY - 2019/5/7
Y1 - 2019/5/7
N2 - Two eye-colour mutant strains, white (W) and yellow (Y) of house cricket Acheta domesticus were established in our laboratory. We phenotyped and genotyped the mutants, performed genetic crossings and studied the eye structure and pigment composition using light and electron microscopy and biochemical analysis. We show that W and Y phenotypes are controlled by a single autosomal recessive allele, as both traits are metabolically independent. The analysis of the mutants'eye structure showed a reduced number of dark pigment granules while simultaneously, and an increased amount of light vacuoles in white eye mutants was observed. Significant differences in eye pigment composition between strains were also found. The Y mutant had a lower number of ommochromes, while the W mutant had a lower number of ommochromes and pteridines. This indicates that mutated genes are involved in two different, independent metabolic pathways regulating tryptophan metabolism enzymes, pigment transporter granules or pigment granule formation.
AB - Two eye-colour mutant strains, white (W) and yellow (Y) of house cricket Acheta domesticus were established in our laboratory. We phenotyped and genotyped the mutants, performed genetic crossings and studied the eye structure and pigment composition using light and electron microscopy and biochemical analysis. We show that W and Y phenotypes are controlled by a single autosomal recessive allele, as both traits are metabolically independent. The analysis of the mutants'eye structure showed a reduced number of dark pigment granules while simultaneously, and an increased amount of light vacuoles in white eye mutants was observed. Significant differences in eye pigment composition between strains were also found. The Y mutant had a lower number of ommochromes, while the W mutant had a lower number of ommochromes and pteridines. This indicates that mutated genes are involved in two different, independent metabolic pathways regulating tryptophan metabolism enzymes, pigment transporter granules or pigment granule formation.
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U2 - 10.1371/JOURNAL.PONE.0216281
DO - 10.1371/JOURNAL.PONE.0216281
M3 - Article
C2 - 31059541
AN - SCOPUS:85065662274
VL - 14
JO - PLoS ONE
JF - PLoS ONE
SN - 1932-6203
IS - 5
M1 - e0216281
ER -