Changing exposure media can reverse the cytotoxicity of ceria nanoparticles for Escherichia coli

Xiao He, Yashu Kuang, Yuanyuan Li, Haifeng Zhang, Yuhui Ma, Wei Bai, Zhiyong Zhang, Zhenqiang Wu, Yuliang Zhao, Zhifang Chai

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Ceria nanoparticles have attracted a great deal of concern about their impact on human health and the environment due to their widespread applications. In the present work, different exposure media (normal saline and phosphate-buffered saline [PBS]) were used to adjust the surface charge density in order to investigate the influence of surface charge on the cytotoxicity of ceria nanoparticles for Escherichia coli. The results showed that the direct contact mediated by the electrostatic attraction between the cell wall and the positive-charged ceria nanoparticles in normal saline would result in outer membrane destabilization, increased reactive oxygen species (ROS) production and loss of viability. The situation in PBS was totally different, with significantly reduced contacts, so no outer membrane destabilization, no increased ROS production, and no cytotoxicity. The results suggested that surface charge density was closely involved in the cytotoxicity of ceria nanoparticles for E. coli. This work as a well designed comparison study contributes to a better understanding of the charge-associated biological effects of nanomaterials.

Original languageEnglish (US)
Pages (from-to)233-240
Number of pages8
JournalNanotoxicology
Volume6
Issue number3
DOIs
StatePublished - May 2012

Keywords

  • Ceria nanoparticles
  • Cytotoxicity
  • Exposure media
  • In situ assessment
  • Surface charge density

ASJC Scopus subject areas

  • Biomedical Engineering
  • Toxicology

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