The conformational memory of enzyme has a significant application in biocatalysis. In this work, pH modification of tannase and the relevant factors were studied. This results indicate that direct addition of pH buffer lyophilized powder in organic media can remarkably change the conformation as did pH tunning, and the conformation variation closely correlates with the balance between hydrophilic solvents and hydrophobic solvents in reaction system. pH tunning of inner tannase and free tannase was performed. The results show that the variation may be affected by its spatial redundancy. It will be potentially applied in biocatalysis in organic media.
- Conformational memory
- pH tunning
ASJC Scopus subject areas
- Process Chemistry and Technology