Changes in the structure of the cell surface carbohydrates of the chinchilla tubotympanum following Streptococcus pneumoniae-induced otitis media

T. E. Linder, David J. Lim, T. F. DeMaria

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

Streptococcus pneumoniae (Spn) are among the most frequently isolated pathogens in acute otitis media (AOM) and in otitis media with effusion (OME). Recently, the specific receptor for Spn has been identified as the trisaccharide unit Galβ1-4 GlcNAcβ1-3 Galβ with GlcNAcβ1-3 Galβ as the principal binding site. During the colonization of mucosal surfaces, pneumococci produce a variety of enzymes. This study was conducted to identify any resulting changes in the cell surface carbohydrate structure due to the action of these enzymes during pneumococcal otitis media (OM) in chinchillas. Using a lectin histochemical method with seven different lectins (SNA, LFA, WGA, Succ WGA, BSL II, PNA, ECL), the labeling pattern revealed not only the removal of the terminal sialic acid, but also the exposure of N-acetyl-glucosamine. These results suggested that Spn-produced enzymes uncover part of their own receptor structure and thus may facilitate adherence and subsequent infection.

Original languageEnglish (US)
Pages (from-to)293-303
Number of pages11
JournalMicrobial Pathogenesis
Volume13
Issue number4
DOIs
StatePublished - Jan 1 1992

Keywords

  • Streptococcus pneumoniae
  • chinchilla
  • eustachian tube
  • glycoconjugates
  • otitis media

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

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