TY - JOUR
T1 - Cellular Responses to Exposure to Outdoor Air from the Chinese Spring Festival at the Air-Liquid Interface
AU - Ding, Jie
AU - Guo, Jincheng
AU - Wang, Liming
AU - Chen, Yandong
AU - Hu, Bin
AU - Li, Yiye
AU - Huang, Rujin
AU - Cao, Junji
AU - Zhao, Yuliang
AU - Geiser, Marianne
AU - Miao, Qing
AU - Liu, Ying
AU - Chen, Chunying
N1 - Funding Information:
This work was financially supported by the National Natural Science Foundation of China (11435002, 91543206, 91543127, and 31622026), the National Basic Research Program of China (2016YFA0201600, 2016YFE0133100, 2017YFC1600204, and 2016YFA0203204), the Science Fund for Creative Research Groups of the National Natural Science Foundation of China (11621505), CAS Key Research Program for Frontier Sciences (QYZDJ-SSW-SLH022), and the National Science Fund for Distinguished Young Scholars (11425520).
Publisher Copyright:
© 2019 American Chemical Society.
PY - 2019/8/6
Y1 - 2019/8/6
N2 - The Spring Festival is the most important holiday in China. During this time, the levels of particulate matter (PM) as well as gaseous copollutants significantly increase because of the widespread enjoyment of fireworks. The expression patterns of microRNAs may serve as valuable signatures of exposure to environmental constituents. We exposed macrophages to the whole stream of outdoor air at the air-liquid interface aiming at closely approximating the physiological conditions and the inhalation situation in the lung. 58 miRNAs were up-regulated, and 68 miRNAs were down-regulated in the night of the New Year's Eve (exposure group E2N1) compared to filtered-air exposed control cells. The target genes of the up-regulated miRNAs were enriched in immunity- and inflammation-linked pathways, such as the TLR-NF-κB pathway. Compared to the E2N1 group, 29 miRNAs were up-regulated, and 23 miRNAs were down-regulated in the cells exposed to air from the daytime of the Chinese New Year with higher concentrations of particles, SO2, and nitrogen oxide. The target genes of the up-regulated miRNAs were mostly enriched in apoptosis, adhesion, and junction-related pathways. These results preliminarily unravel part of the toxic mechanisms of air constituents and provide clues for discovering the main drivers of air pollution-induced disorders.
AB - The Spring Festival is the most important holiday in China. During this time, the levels of particulate matter (PM) as well as gaseous copollutants significantly increase because of the widespread enjoyment of fireworks. The expression patterns of microRNAs may serve as valuable signatures of exposure to environmental constituents. We exposed macrophages to the whole stream of outdoor air at the air-liquid interface aiming at closely approximating the physiological conditions and the inhalation situation in the lung. 58 miRNAs were up-regulated, and 68 miRNAs were down-regulated in the night of the New Year's Eve (exposure group E2N1) compared to filtered-air exposed control cells. The target genes of the up-regulated miRNAs were enriched in immunity- and inflammation-linked pathways, such as the TLR-NF-κB pathway. Compared to the E2N1 group, 29 miRNAs were up-regulated, and 23 miRNAs were down-regulated in the cells exposed to air from the daytime of the Chinese New Year with higher concentrations of particles, SO2, and nitrogen oxide. The target genes of the up-regulated miRNAs were mostly enriched in apoptosis, adhesion, and junction-related pathways. These results preliminarily unravel part of the toxic mechanisms of air constituents and provide clues for discovering the main drivers of air pollution-induced disorders.
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U2 - 10.1021/acs.est.9b00399
DO - 10.1021/acs.est.9b00399
M3 - Article
C2 - 31268311
AN - SCOPUS:85071063847
SN - 0013-936X
VL - 53
SP - 9128
EP - 9138
JO - Environmental Science and Technology
JF - Environmental Science and Technology
IS - 15
ER -