Cell growth inhibition by prostaglandin A2 results in elevated expression of gadd153 mRNA

Augustine M K Choi , Joseph Fargnoli, Sara G. Carlson, Nikki J. Holbrook

Research output: Contribution to journalArticle

43 Scopus citations

Abstract

Treatment of Hela cells with prostaglandin A2 (PGA2) resulted in a marked inhibition of cell proliferation which was associated with a significant induction of gadd153 mRNA, a member of a novel class of genes associated with growth arrest and DNA damage. Induction of gadd153 mRNA was specific to prostaglandins capable of arresting cell growth and was dose-dependent with the maximum effect seen at 36 μM PGA2. Induction was rapid, occurring within 2-4 h and reaching a maximum by 8 h. These effects were reversible as removal of PGA2 resulted in a rapid decline in gadd153 mRNA levels coincident with resumption of cell growth. PGA2 induction of gadd153 mRNA was completely prevented by the presence of actinomycin D at a concentration sufficient to block transcription and was partially inhibited (50%) by the protein synthesis inhibitor cycloheximide. The presence of the protein kinase inhibitor 2-aminopurine decreased the PGA2 induction of gadd153 mRNA by greater than 90%, suggesting that cellular kinases play a role in the induction of gadd153 by PGA2. Thus PGA2-mediated growth arrest provides a useful model to further define the role of gadd153 in the negative control of cell growth.

Original languageEnglish (US)
Pages (from-to)85-89
Number of pages5
JournalExperimental Cell Research
Volume199
Issue number1
DOIs
StatePublished - Jan 1 1992

ASJC Scopus subject areas

  • Cell Biology

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