Cell discrimination among simultaneous presentation of substrate-bound and soluble protein gradients

S. G. Ricoult; D. J. Lee; A. S. Ravan; Amy Q. Shen

Cell discrimination among simultaneous presentation of substrate-bound and soluble protein gradients

S. G. Ricoult, D. J. Lee, A. S. Ravan, Amy Q. Shen

Houston Methodist

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

To overcome the extracellular matrix's (ECM) complexity, cells rely on a plethora of cues to reach specific targets. Cues are concurrently presented to cells, and the cells must discriminate amongst these cues through a mechanism yet to be understood. To study cell navigation, in vitro methods have been developed to create gradients of single cues, such as substrate-bound or soluble proteins. Although insightful, these approaches lack the ability to present cells with multiple cues of various types. Here, we present a platform which for the first time enables the study of cell discrimination between soluble and substrate-bound protein gradients.

Original language	English (US)
Title of host publication	MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Publisher	Chemical and Biological Microsystems Society
Pages	680-682
Number of pages	3
ISBN (Electronic)	9780979806483
State	Published - 2015
Event	19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of Duration: Oct 25 2015 → Oct 29 2015

Other

Other	19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
Country/Territory	Korea, Republic of
City	Gyeongju
Period	10/25/15 → 10/29/15

Keywords

Cell navigation
Chemotaxis
Digital nanodot gradient
Haptotaxis
Protein gradient

ASJC Scopus subject areas

Control and Systems Engineering

Cite this

Cell discrimination among simultaneous presentation of substrate-bound and soluble protein gradients. / Ricoult, S. G.; Lee, D. J.; Ravan, A. S. et al.
MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2015. p. 680-682.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Ricoult, SG, Lee, DJ, Ravan, AS & Shen, AQ 2015, Cell discrimination among simultaneous presentation of substrate-bound and soluble protein gradients. in MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, pp. 680-682, 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015, Gyeongju, Korea, Republic of, 10/25/15.

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abstract = "To overcome the extracellular matrix's (ECM) complexity, cells rely on a plethora of cues to reach specific targets. Cues are concurrently presented to cells, and the cells must discriminate amongst these cues through a mechanism yet to be understood. To study cell navigation, in vitro methods have been developed to create gradients of single cues, such as substrate-bound or soluble proteins. Although insightful, these approaches lack the ability to present cells with multiple cues of various types. Here, we present a platform which for the first time enables the study of cell discrimination between soluble and substrate-bound protein gradients.",

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AU - Lee, D. J.

AU - Ravan, A. S.

AU - Shen, Amy Q.

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Y1 - 2015

N2 - To overcome the extracellular matrix's (ECM) complexity, cells rely on a plethora of cues to reach specific targets. Cues are concurrently presented to cells, and the cells must discriminate amongst these cues through a mechanism yet to be understood. To study cell navigation, in vitro methods have been developed to create gradients of single cues, such as substrate-bound or soluble proteins. Although insightful, these approaches lack the ability to present cells with multiple cues of various types. Here, we present a platform which for the first time enables the study of cell discrimination between soluble and substrate-bound protein gradients.

AB - To overcome the extracellular matrix's (ECM) complexity, cells rely on a plethora of cues to reach specific targets. Cues are concurrently presented to cells, and the cells must discriminate amongst these cues through a mechanism yet to be understood. To study cell navigation, in vitro methods have been developed to create gradients of single cues, such as substrate-bound or soluble proteins. Although insightful, these approaches lack the ability to present cells with multiple cues of various types. Here, we present a platform which for the first time enables the study of cell discrimination between soluble and substrate-bound protein gradients.

KW - Cell navigation

KW - Chemotaxis

KW - Digital nanodot gradient

KW - Haptotaxis

KW - Protein gradient

UR - https://www.scopus.com/pages/publications/84983372839

UR - https://www.scopus.com/inward/citedby.url?scp=84983372839&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:84983372839

SP - 680

EP - 682

BT - MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences

PB - Chemical and Biological Microsystems Society

T2 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015

Y2 - 25 October 2015 through 29 October 2015

ER -

Cell discrimination among simultaneous presentation of substrate-bound and soluble protein gradients

Abstract

Other

Keywords

ASJC Scopus subject areas

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