Cell cycle regulation of c-Jun N-terminal kinase activity at the centrosomes

Rebecca MacCorkle-Chosnek, Aaron VanHooser, David W. Goodrich, B. R. Brinkley, Tse Hua Tan

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

The c-Jun N-terminal kinase (JNK), a subgroup of the mitogen-activated protein kinase (MAPK) family of serine/threonine kinases, has established functions in cell growth and apoptosis. While the mechanisms are unclear, JNK has also been also implicated in signaling pathways that initiate cell cycle checkpoints and cell cycle progression. By following the localization of active and inactive JNK during the cell cycle, we have found that the majority of cellular JNK is soluble and present in the cytoplasm and the nucleus. Interestingly, insoluble fractions of JNK are also localized in nuclear and cytoplasmic speckles, and to the centrosomes. While JNK is associated with the centrosome throughout the cell cycle, it is only active at the centrosome from S phase through anaphase. This novel localization of centrosomal JNK is a possible link between JNK-activating stimuli and centrosome or cell cycle events.

Original languageEnglish (US)
Pages (from-to)173-180
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume289
Issue number1
DOIs
StatePublished - Nov 23 2001

Keywords

  • Cell cycle
  • Centrosome
  • Immunofluorescence
  • JNK
  • Kinase
  • Localization
  • Pericentriolar matrix

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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