Cell cycle-dependent modulation of o-methylguanine-dna methyltransferse in c3h/10t1/2 cells

William C. Dunn, Robert S. Foote, Russ E. Hand, Sankar Mitra

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

O6-methylguanine-DNA methyltransferase (MGMT) was measured in partially synchronized cultures of C3H/10T1/2 mouse embryo cells as a function of cell cycle. The degree of synchrony and progression of the cell cycle were monitored by flow cytometry. The MGMT level was significantly reduced prior to the onset of S-phase. This reduction was concomitant with the inhibition of in vivo repair of O6-methylguanine in DNA of S-phase cells as observed earlier. The recovery of the MGMT level paralleled the progression of synchronized cells into G2. S-phase cells purified by cell sorting contained -15% of the MGMT present in Go or early G1 cells. A comparison of the in vivo repair of O6-methylguanine and MGMT levels suggests that the lack of repair of O6-methylguanine in DNA of the mouse embryo cells is due only in part to a temporal loss of MGMT.

Original languageEnglish (US)
Pages (from-to)807-812
Number of pages6
JournalCarcinogenesis
Volume7
Issue number5
DOIs
StatePublished - May 1986

ASJC Scopus subject areas

  • Cancer Research

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