Abstract
O6-methylguanine-DNA methyltransferase (MGMT) was measured in partially synchronized cultures of C3H/10T1/2 mouse embryo cells as a function of cell cycle. The degree of synchrony and progression of the cell cycle were monitored by flow cytometry. The MGMT level was significantly reduced prior to the onset of S-phase. This reduction was concomitant with the inhibition of in vivo repair of O6-methylguanine in DNA of S-phase cells as observed earlier. The recovery of the MGMT level paralleled the progression of synchronized cells into G2. S-phase cells purified by cell sorting contained -15% of the MGMT present in Go or early G1 cells. A comparison of the in vivo repair of O6-methylguanine and MGMT levels suggests that the lack of repair of O6-methylguanine in DNA of the mouse embryo cells is due only in part to a temporal loss of MGMT.
Original language | English (US) |
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Pages (from-to) | 807-812 |
Number of pages | 6 |
Journal | Carcinogenesis |
Volume | 7 |
Issue number | 5 |
DOIs | |
State | Published - May 1986 |
ASJC Scopus subject areas
- Cancer Research