Cell binding specificity of mouse R-cadherin and chromosomal mapping of the gene

H. Matsunami, S. Miyatani, T. Inoue, Neal G. Copeland, D. J. Gilbert, Nancy A. Jenkins, M. Takeichi

Research output: Contribution to journalArticle

81 Scopus citations

Abstract

R-cadherin was originally identified as a chicken cadherin expressed by the retina. Here, we describe the identification of a mouse homologue of R-cadherin. We isolated mouse cDNAs encoding a cadherin with 94% identity in amino acid sequence to the chicken R-cadherin, and defined this molecule as mouse R-cadherin. L cell transfected with the mouse R-cadherin cDNA acquired a cadherin-mediated cell-cell adhesiveness as found for other cadherins. To examine the binding specificity of mouse R-cadherin, L cells expressing this cadherin (mRL) were mixed with L cells expressing chicken R-cadherin (cRL), mouse N-cadherin (mNL), mouse E-cadherin (mEL) and mouse P-cadherin (mPL). While mRL cells randomly intermixed with cRL cells, those cells aggregated separately from mEL or mPL cells. Mixing of mRL with mNL cells gave an intermediate result; that is, they formed both separate and chimeric aggregates, suggesting that R- and N-cadherin can interact with each other although each has a preference to bind to its own type. Similar properties were previously found for chicken R-cadherin. Thus, the cell binding specificity of R-cadherin is entirely conserved between the two species, suggesting a conserved role for this protein in morphogenesis. We also located the mouse R-cadherin gene to chromosome 2.

Original languageEnglish (US)
Pages (from-to)401-409
Number of pages9
JournalJournal of Cell Science
Volume106
Issue number1
StatePublished - Jan 1 1993

Keywords

  • Cadherins
  • Cell adhesion
  • Cell sorting
  • Chromosomal mapping

ASJC Scopus subject areas

  • Cell Biology

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