TY - JOUR
T1 - Cell attachment to frozen sections of injured adult mouse brain
T2 - Effects of tenascin antibody and lectin perturbation of wound-related extracellular matrix molecules
AU - Laywell, Eric D.
AU - Friedman, Paul
AU - Harrington, Kristy
AU - Robertson, James T.
AU - Steindler, Dennis A.
N1 - Funding Information:
The authors thank Donna Gates for expert technical assistance. This work was supported by NIH/NINDS grant NS29225 (D.A.S.), a grant from the Baptist Memorial Hospital Research Foundation (J.T.R., D.A.S., E.D.L.), and NIH Medical Student Research Fellowship (T3SDK07405) from the UT Memphis College of Medicine (P.F. ).
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1996/6
Y1 - 1996/6
N2 - Previous studies describing the use of cryoculture methods have focused on the efficacy of the method for studying neuron attachment and neurite outgrowth on intact sections of nerve, and rodent and even human brain. The cryoculture method has shown promise for determining the presence of cell attachment and neurite-growth-inhibiting molecules in such specimens, and some studies have also attempted to neutralize such molecules with antibodies to myelin inhibitory proteins, nerve growth factor, or factors present in conditioned media that may counteract the repulsiveness of some of these molecules preserved in sections of for example, myelinated nerves or adult brain white matter. The present study describes the novel use of lesioned central nervous system cryocultures as substrates for investigating the attachment of embryonic neurons and PC12 cells. In addition to demonstrating the use of this novel scar substrate to extend previous 'scar-in-a-dish' models (David et al. (1990) Neuron, 5: 463-469: Rudge and Silver (1990) J. Neurosci., 10: 3594-3603; Rudge et al. (1989) Exp. Neurol., 103: 1-16), the present study also describes antibody and lectin perturbations of putative inhibitory molecules that result in an enhanced attachment of cells to cryosection cultures of brain and spinal cord wounds.
AB - Previous studies describing the use of cryoculture methods have focused on the efficacy of the method for studying neuron attachment and neurite outgrowth on intact sections of nerve, and rodent and even human brain. The cryoculture method has shown promise for determining the presence of cell attachment and neurite-growth-inhibiting molecules in such specimens, and some studies have also attempted to neutralize such molecules with antibodies to myelin inhibitory proteins, nerve growth factor, or factors present in conditioned media that may counteract the repulsiveness of some of these molecules preserved in sections of for example, myelinated nerves or adult brain white matter. The present study describes the novel use of lesioned central nervous system cryocultures as substrates for investigating the attachment of embryonic neurons and PC12 cells. In addition to demonstrating the use of this novel scar substrate to extend previous 'scar-in-a-dish' models (David et al. (1990) Neuron, 5: 463-469: Rudge and Silver (1990) J. Neurosci., 10: 3594-3603; Rudge et al. (1989) Exp. Neurol., 103: 1-16), the present study also describes antibody and lectin perturbations of putative inhibitory molecules that result in an enhanced attachment of cells to cryosection cultures of brain and spinal cord wounds.
KW - Antibody perturbation
KW - Brain wound
KW - Cryoculture
KW - In vitro bioassay
KW - Lectin perturbation
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U2 - 10.1016/0165-0270(96)00008-8
DO - 10.1016/0165-0270(96)00008-8
M3 - Article
C2 - 8835793
AN - SCOPUS:0030176143
VL - 66
SP - 99
EP - 108
JO - Journal of Neuroscience Methods
JF - Journal of Neuroscience Methods
SN - 0165-0270
IS - 2
ER -