TY - JOUR
T1 - cDNA cloning, expression, subcellular localization, and chromosomal assignment of mammalian aurora homologues, aurora-related kinase (ARK) 1 and 2
AU - Shindo, Masahisa
AU - Nakano, Hiroyasu
AU - Kuroyanagi, Hidehito
AU - Shirasawa, Takuji
AU - Mihara, Motoyuki
AU - Gilbert, Debra J.
AU - Jenkins, Nancy A.
AU - Copeland, Neal G.
AU - Yagita, Hideo
AU - Okumura, Ko
N1 - Funding Information:
We thank Drs. Shoichiro Miyatake, Hiroshi Ohno, Takumi Akashi, and Hiroshi Itoh for helpful discussion and reagents. We also thank Mary Barnstead and Andree Reuss for excellent technical assistance. This work was supported by grants from the Ministry of Education, Science, and Culture; the Ministry of Health, Japan; Uehara Memorial Foundation; and in part by the National Cancer Institute, DHHS, under contract with ABL.
PY - 1998/3/6
Y1 - 1998/3/6
N2 - Chromosomal segregation during mitosis as well as meiosis is considered to be regulated by multiple kinases, but the precise mechanism remains largely unknown. A mutation in Drosophila, designated aurora, was identified as a responsible gene for a chromosomal segregation defect and encodes a putative serine-threonine kinase. Here we have identified mammalian aurora homologues, designated aurora-related kinase (ARK) 1 and ARK2. Kinase domains of murine ARK1 and ARK2 showed 61 and 62% identity, respectively, to that of aurora at the amino acid levels, respectively. Cell cycle analysis revealed that the expression of ARK1 was correlated with G2/M phase, while ARK2 was expressed during S and G2/M phases. Immunofluorescence analysis demonstrated that ARK2 was mainly localized to the midbody, while ARK1 has been reported to be localized to the spindle pole during mitosis. Collectively, these results suggest that these two kinases may have distinct roles with different expression timing and subcellular localization during the cell cycle progression. Interspecific backcross mapping revealed that Ark1 is located in a distal region of mouse chromosome 2, while Ark2 is located in a central region of mouse chromosome 11.
AB - Chromosomal segregation during mitosis as well as meiosis is considered to be regulated by multiple kinases, but the precise mechanism remains largely unknown. A mutation in Drosophila, designated aurora, was identified as a responsible gene for a chromosomal segregation defect and encodes a putative serine-threonine kinase. Here we have identified mammalian aurora homologues, designated aurora-related kinase (ARK) 1 and ARK2. Kinase domains of murine ARK1 and ARK2 showed 61 and 62% identity, respectively, to that of aurora at the amino acid levels, respectively. Cell cycle analysis revealed that the expression of ARK1 was correlated with G2/M phase, while ARK2 was expressed during S and G2/M phases. Immunofluorescence analysis demonstrated that ARK2 was mainly localized to the midbody, while ARK1 has been reported to be localized to the spindle pole during mitosis. Collectively, these results suggest that these two kinases may have distinct roles with different expression timing and subcellular localization during the cell cycle progression. Interspecific backcross mapping revealed that Ark1 is located in a distal region of mouse chromosome 2, while Ark2 is located in a central region of mouse chromosome 11.
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U2 - 10.1006/bbrc.1998.8250
DO - 10.1006/bbrc.1998.8250
M3 - Article
C2 - 9514916
AN - SCOPUS:0032489332
SN - 0006-291X
VL - 244
SP - 285
EP - 292
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -