CD30 antigen, a marker for Hodgkin's lymphoma, is a receptor whose ligand defines an emerging family of cytokines with homology to TNF

Craig A. Smith, Hans Juergen Gruss, Terri Davis, Dirk Anderson, Terry Farrah, Elizabeth Baker, Grant R. Sutherland, Camilynn I. Brannan, Neal G. Copeland, Nancy A. Jenkins, Kenneth H. Grabstein, Brian Gliniak, Ian B. McAlister, William Fanslow, Mark Alderson, Ben Falk, Steve Gimpel, Steven Gillis, Wenie S. Din, Raymond G. GoodwinRichard J. Armitage

Research output: Contribution to journalArticle

493 Scopus citations

Abstract

CD30 is a surface marker for neoplastic cells of Hodgkin's lymphoma and shows sequence homology to members of the tumor necrosis factor (TNF) receptor superfamily. Using a chimeric probe consisting of the extracellular domain of CD30 fused to truncated immunoglobulin heavy chains, we expression cloned the cDNA cognate from the murine T cell clone 7B9. The encoded protein is a 239 amino acid type II membrane protein whose C-terminal domain shows significant homology to TNFα, TNFβ, and the CD40L. Cross-hybridization to an induced peripheral blood T cell cDNA library yielded the human homolog, which is 72% identical at the amino acid level. The recombinant human ligand enhances the proliferation of CD3-activated T cells yet induces differential responses, including cell death, in several CD30+ lymphoma-derived clones. The human and murine genes map to 9q33 and the proximal region of chromosome 4, respectively.

Original languageEnglish (US)
Pages (from-to)1349-1360
Number of pages12
JournalCell
Volume73
Issue number7
DOIs
StatePublished - Jul 2 1993

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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