CD3 mAb treatment ameliorated the severity of the cGVHD-induced lupus nephritis in mice by up-regulation of Foxp3 + regulatory T cells in the target tissue: Kidney

Ji Lu Zhang, De Jun Sun, Chun Mei Hou, Ying Lin Wei, Xin Ying Li, Zu Yin Yu, Jian Nan Feng, Bei Fen Shen, Yan Li, He Xiao

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Teff/Treg imbalance orchestrated the onset and the progression of the lupus nephritis in a DBA/2→B6D2F1 murine model with cGVHD. In this paper, we first used 145-2C11 Ab to treat these human SLE-like diseased animals. The results showed that short-term low-dose anti-CD3 antibody treatment induced a significant remission of established proteinuria, production of autoantibodies, immune complex deposition and renal parenchyma lesions in lupus nephritic mice. Of note, we found a robust up-regulation of Foxp3 mRNA expression in the target tissue: kidney from mice with anti-CD3 antibody treatment compared to those with control IgG treatment. Likewise, an increased renal mRNA abundance for IL-10 was also observed in anti-CD3 antibody treated mice. In contrast, genes associated with inflammation and fibrosis as well as cytokines related to effector T cell responses were down-regulated by anti-CD3 mAb treatment. These findings suggested that short-term low-dose anti-CD3 antibody treatment might induced an IL-10-secreting Foxp3 + regulatory T cells in this cGVHD target tissue: kidney, that suppressed the activation of effector T cells (Th1, Th2 and Th17), thus ameliorating the severity of the lupus nephritis in mice.

Original languageEnglish (US)
Pages (from-to)17-25
Number of pages9
JournalTransplant Immunology
Volume24
Issue number1
DOIs
StatePublished - Oct 2010

Keywords

  • Anti-CD3 mAb
  • CGVHD
  • Foxp3
  • Lupus nephritis
  • Teff/Treg balance
  • Up-regulation

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Transplantation

Fingerprint Dive into the research topics of 'CD3 mAb treatment ameliorated the severity of the cGVHD-induced lupus nephritis in mice by up-regulation of Foxp3 <sup>+</sup> regulatory T cells in the target tissue: Kidney'. Together they form a unique fingerprint.

Cite this