Caspase activation in response to cytotoxic Rana catesbeiana ribonuclease in MCF-7 cells

Chih Chi Andrew Hu; Chih Hang Anthony Tang; Jaang Jiun Wang

doi:10.1016/S0014-5793(01)02691-6

Caspase activation in response to cytotoxic Rana catesbeiana ribonuclease in MCF-7 cells

Chih Chi Andrew Hu, Chih Hang Anthony Tang, Jaang Jiun Wang

Research output: Contribution to journal › Article › peer-review

44 Scopus citations

Abstract

Rana catesbeiana ribonuclease (RC-RNase) and onconase were proven to own anti-tumor activity. While molecular determinants of onconase-induced cell death have become more explicit, the RC-RNase-induced death pathway remains presently unknown. Here we demonstrated that RC-RNase-induced molecular cascades in caspase-3-deficient MCF-7 cells did not include activation of initiation caspase-8 and -9. Cleavage timing suggested that procaspase-2 and -6 might be processed by active caspase-7 in MCF-7 cells. Caspase-7 was also responsible for cleavage of the poly(ADP-ribose) polymerase. Furthermore, we reported that overexpression of Bcl-X_L could raise the survival rates of MCF-7 cells treated with RC-RNase and onconase.

Original language	English (US)
Pages (from-to)	65-68
Number of pages	4
Journal	FEBS Letters
Volume	503
Issue number	1
DOIs	https://doi.org/10.1016/S0014-5793(01)02691-6
State	Published - Aug 10 2001

Keywords

Bcl-X
Caspase
MCF-7
Onconase
Rana catesbeiana ribonuclease

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(01)02691-6

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@article{006ff76b089e443dba5617cb34c4c976,

title = "Caspase activation in response to cytotoxic Rana catesbeiana ribonuclease in MCF-7 cells",

abstract = "Rana catesbeiana ribonuclease (RC-RNase) and onconase were proven to own anti-tumor activity. While molecular determinants of onconase-induced cell death have become more explicit, the RC-RNase-induced death pathway remains presently unknown. Here we demonstrated that RC-RNase-induced molecular cascades in caspase-3-deficient MCF-7 cells did not include activation of initiation caspase-8 and -9. Cleavage timing suggested that procaspase-2 and -6 might be processed by active caspase-7 in MCF-7 cells. Caspase-7 was also responsible for cleavage of the poly(ADP-ribose) polymerase. Furthermore, we reported that overexpression of Bcl-XL could raise the survival rates of MCF-7 cells treated with RC-RNase and onconase.",

keywords = "Bcl-X, Caspase, MCF-7, Onconase, Rana catesbeiana ribonuclease",

author = "Hu, {Chih Chi Andrew} and Tang, {Chih Hang Anthony} and Wang, {Jaang Jiun}",

note = "Funding Information: This research was partially sponsored by a grant from the National Science Council, R.O.C. (NSC 89-2320-B-016-109). The authors are grateful to Dr. Kuslima Shogen for her generous supply of onconase and to Dr. You-Di Liao for he was always one of our best collaborators. ",

year = "2001",

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day = "10",

doi = "10.1016/S0014-5793(01)02691-6",

language = "English (US)",

volume = "503",

pages = "65--68",

journal = "FEBS Letters",

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TY - JOUR

T1 - Caspase activation in response to cytotoxic Rana catesbeiana ribonuclease in MCF-7 cells

AU - Hu, Chih Chi Andrew

AU - Tang, Chih Hang Anthony

AU - Wang, Jaang Jiun

N1 - Funding Information: This research was partially sponsored by a grant from the National Science Council, R.O.C. (NSC 89-2320-B-016-109). The authors are grateful to Dr. Kuslima Shogen for her generous supply of onconase and to Dr. You-Di Liao for he was always one of our best collaborators.

PY - 2001/8/10

Y1 - 2001/8/10

N2 - Rana catesbeiana ribonuclease (RC-RNase) and onconase were proven to own anti-tumor activity. While molecular determinants of onconase-induced cell death have become more explicit, the RC-RNase-induced death pathway remains presently unknown. Here we demonstrated that RC-RNase-induced molecular cascades in caspase-3-deficient MCF-7 cells did not include activation of initiation caspase-8 and -9. Cleavage timing suggested that procaspase-2 and -6 might be processed by active caspase-7 in MCF-7 cells. Caspase-7 was also responsible for cleavage of the poly(ADP-ribose) polymerase. Furthermore, we reported that overexpression of Bcl-XL could raise the survival rates of MCF-7 cells treated with RC-RNase and onconase.

AB - Rana catesbeiana ribonuclease (RC-RNase) and onconase were proven to own anti-tumor activity. While molecular determinants of onconase-induced cell death have become more explicit, the RC-RNase-induced death pathway remains presently unknown. Here we demonstrated that RC-RNase-induced molecular cascades in caspase-3-deficient MCF-7 cells did not include activation of initiation caspase-8 and -9. Cleavage timing suggested that procaspase-2 and -6 might be processed by active caspase-7 in MCF-7 cells. Caspase-7 was also responsible for cleavage of the poly(ADP-ribose) polymerase. Furthermore, we reported that overexpression of Bcl-XL could raise the survival rates of MCF-7 cells treated with RC-RNase and onconase.

KW - Bcl-X

KW - Caspase

KW - MCF-7

KW - Onconase

KW - Rana catesbeiana ribonuclease

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JO - FEBS Letters

JF - FEBS Letters

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ER -

Caspase activation in response to cytotoxic Rana catesbeiana ribonuclease in MCF-7 cells

Abstract

Keywords

ASJC Scopus subject areas

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