Caspase activation in response to cytotoxic Rana catesbeiana ribonuclease in MCF-7 cells

Chih Chi Andrew Hu, Chih Hang Anthony Tang, Jaang Jiun Wang

Research output: Contribution to journalArticle

43 Scopus citations

Abstract

Rana catesbeiana ribonuclease (RC-RNase) and onconase were proven to own anti-tumor activity. While molecular determinants of onconase-induced cell death have become more explicit, the RC-RNase-induced death pathway remains presently unknown. Here we demonstrated that RC-RNase-induced molecular cascades in caspase-3-deficient MCF-7 cells did not include activation of initiation caspase-8 and -9. Cleavage timing suggested that procaspase-2 and -6 might be processed by active caspase-7 in MCF-7 cells. Caspase-7 was also responsible for cleavage of the poly(ADP-ribose) polymerase. Furthermore, we reported that overexpression of Bcl-XL could raise the survival rates of MCF-7 cells treated with RC-RNase and onconase.

Original languageEnglish (US)
Pages (from-to)65-68
Number of pages4
JournalFEBS Letters
Volume503
Issue number1
DOIs
StatePublished - Aug 10 2001

Keywords

  • Bcl-X
  • Caspase
  • MCF-7
  • Onconase
  • Rana catesbeiana ribonuclease

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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