Abstract
Rana catesbeiana ribonuclease (RC-RNase) and onconase were proven to own anti-tumor activity. While molecular determinants of onconase-induced cell death have become more explicit, the RC-RNase-induced death pathway remains presently unknown. Here we demonstrated that RC-RNase-induced molecular cascades in caspase-3-deficient MCF-7 cells did not include activation of initiation caspase-8 and -9. Cleavage timing suggested that procaspase-2 and -6 might be processed by active caspase-7 in MCF-7 cells. Caspase-7 was also responsible for cleavage of the poly(ADP-ribose) polymerase. Furthermore, we reported that overexpression of Bcl-XL could raise the survival rates of MCF-7 cells treated with RC-RNase and onconase.
Original language | English (US) |
---|---|
Pages (from-to) | 65-68 |
Number of pages | 4 |
Journal | FEBS Letters |
Volume | 503 |
Issue number | 1 |
DOIs | |
State | Published - Aug 10 2001 |
Keywords
- Bcl-X
- Caspase
- MCF-7
- Onconase
- Rana catesbeiana ribonuclease
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology