Cardiomyocyte Maturation Requires TLR3 Activated Nuclear Factor Kappa B

Conrad P. Hodgkinson, Richard E. Pratt, Imke Kirste, Sophie Dal-Pra, John P. Cooke, Victor J. Dzau

Research output: Contribution to journalArticlepeer-review

31 Scopus citations


The process by which committed precursors mature into cardiomyocytes is poorly understood. We found that TLR3 inhibition blocked cardiomyocyte maturation; precursor cells committed to the cardiomyocyte lineage failed to express maturation genes and sarcomeres did not develop. Using various approaches, we found that the effects of TLR3 upon cardiomyocyte maturation were dependent upon the RelA subunit of nuclear factor kappa B (NFκB). Importantly, under conditions that promote the development of mature cardiomyocytes NFκB became significantly enriched at the promoters of cardiomyocyte maturation genes. Furthermore, activation of the TLR3-NFκB pathway enhanced cardiomyocyte maturation. This study, therefore, demonstrates that the TLR3-NFκB pathway is necessary for the maturation of committed precursors into mature cardiomyocytes. Stem Cells 2018;36:1198–1209.

Original languageEnglish (US)
Pages (from-to)1198-1209
Number of pages12
Issue number8
StatePublished - Aug 2018


  • Cardiac reprogramming
  • Cardiomyocyte development
  • Cardiomyocyte maturation
  • Innate immunity
  • MicroRNAs
  • Nuclear factor kappa B
  • TLR3

ASJC Scopus subject areas

  • Molecular Medicine
  • Developmental Biology
  • Cell Biology


Dive into the research topics of 'Cardiomyocyte Maturation Requires TLR3 Activated Nuclear Factor Kappa B'. Together they form a unique fingerprint.

Cite this