Carboxyl-functionalized superparamagnetic Fe 3O 4/Poly(St-co-MPS)/SiO 2 composite particles for rapid and sensitive immunoassay

Hang Yuan; Feng Yan; Lan Ma; Feng Wu; Jiaqi Zhuang; Wensheng Yang

doi:10.1166/jnn.2011.3115

Carboxyl-functionalized superparamagnetic Fe ₃O ₄/Poly(St-co-MPS)/SiO ₂ composite particles for rapid and sensitive immunoassay

Hang Yuan, Feng Yan, Lan Ma, Feng Wu, Jiaqi Zhuang, Wensheng Yang

Houston Methodist

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Superparamagnetic Fe ₃O ₄/poly(St-co-MPS)/SiO ₂ composite particles with the average size of 140 nm were functionalized with carboxyl group by emulsion polymerization. Functionalized particles with carboxyl contents of 13.6 and 136 μmol/mL were prepared by changing the amount of acrylic acid monomer used in the polymerization. After conjugation with human chorionic gonadotrophin (hCG) antibody, the particles were used to construct lateral flow immunoassays (LFIA) for the detection of hCG in solution. The quantitative analysis could be finished in 20 min by using the magnetic particles as labels. The detection limit of LFIA was determined to be 1 and 5 IU/L respectively for the magnetic particles with carboxyl contents of 13.6 and 136 μmol/mL.

Original language	English (US)
Pages (from-to)	2232-2236
Number of pages	5
Journal	Journal of Nanoscience and Nanotechnology
Volume	11
Issue number	3
DOIs	https://doi.org/10.1166/jnn.2011.3115
State	Published - Mar 2011

Keywords

Carboxyl group
Lateral flow immunoassay
Sensitivity
Superparamagnetic particles

ASJC Scopus subject areas

Condensed Matter Physics
General Chemistry
General Materials Science
Bioengineering
Biomedical Engineering

Access to Document

10.1166/jnn.2011.3115

Cite this

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title = "Carboxyl-functionalized superparamagnetic Fe 3O 4/Poly(St-co-MPS)/SiO 2 composite particles for rapid and sensitive immunoassay",

abstract = "Superparamagnetic Fe 3O 4/poly(St-co-MPS)/SiO 2 composite particles with the average size of 140 nm were functionalized with carboxyl group by emulsion polymerization. Functionalized particles with carboxyl contents of 13.6 and 136 μmol/mL were prepared by changing the amount of acrylic acid monomer used in the polymerization. After conjugation with human chorionic gonadotrophin (hCG) antibody, the particles were used to construct lateral flow immunoassays (LFIA) for the detection of hCG in solution. The quantitative analysis could be finished in 20 min by using the magnetic particles as labels. The detection limit of LFIA was determined to be 1 and 5 IU/L respectively for the magnetic particles with carboxyl contents of 13.6 and 136 μmol/mL.",

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AU - Yan, Feng

AU - Ma, Lan

AU - Wu, Feng

AU - Zhuang, Jiaqi

AU - Yang, Wensheng

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AB - Superparamagnetic Fe 3O 4/poly(St-co-MPS)/SiO 2 composite particles with the average size of 140 nm were functionalized with carboxyl group by emulsion polymerization. Functionalized particles with carboxyl contents of 13.6 and 136 μmol/mL were prepared by changing the amount of acrylic acid monomer used in the polymerization. After conjugation with human chorionic gonadotrophin (hCG) antibody, the particles were used to construct lateral flow immunoassays (LFIA) for the detection of hCG in solution. The quantitative analysis could be finished in 20 min by using the magnetic particles as labels. The detection limit of LFIA was determined to be 1 and 5 IU/L respectively for the magnetic particles with carboxyl contents of 13.6 and 136 μmol/mL.

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