Carbon monoxide negatively regulates NLRP3 inflammasome activation in macrophages

Sung Soo Jung, Jong Seok Moon, Jin Fu Xu, Emeka Ifedigbo, Stefan W. Ryter, Augustine M.K. Choi, Kiichi Nakahira

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

Inflammasomes are cytosolic protein complexes that promote the cleavage of caspase-1, which leads to the maturation and secretion of proinflammatory cytokines, including interleukin-1β (IL-1β) and IL-18. Among the known inflammasomes, the nucleotide-binding domain, leucine-rich-containing family, pyrin domain-containing-3 (NLRP3)-dependent inflammasome is critically involved in the pathogenesis of various acute or chronic inflammatory diseases. Carbon monoxide (CO), a gaseous molecule physiologically produced in cells and tissues during heme catabolism, can act as an anti-inflammatory molecule and a potent negative regulator of Tolllike receptor signaling pathways. To date, the role of CO in inflam-masome-mediated immune responses has not been fully investigated. Here, we demonstrated that CO inhibited caspase-1 activation and the secretion of IL-1β and IL-18 in response to lipopolysaccharide (LPS) and ATP treatment in bone marrow-derived macrophages. CO also inhibited IL-18 secretion in response to LPS and nigericin treatment, another NLRP3 inflammasome activation model. In contrast, CO did not suppress IL-18 secretion in response to LPS and poly(dA:dT), an absent in melanoma 2 (AIM2)-mediated inflammasome model. LPS and ATP stimulation induced the formation of complexes between NLRP3 and apoptosis-associated speck-like protein, or NLRP3 and caspase-1. CO treatment inhibited these molecular interactions that were induced by LPS and ATP. Furthermore, CO inhibited mitochondrial ROS generation and the decrease of mitochondrial membrane potential induced by LPS and ATP in macrophages. We also observed that the inhibitory effect of CO on the translocation of mitochondrial DNA into the cytosol was associated with suppression of cytokine secretion. Our results suggest that CO negatively regulates NLRP3 inflammasome activation by preventing mitochondrial dysfunction.

Original languageEnglish (US)
Pages (from-to)L1058-L1067
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume308
Issue number10
DOIs
StatePublished - May 15 2015

Keywords

  • Interleukin-18
  • Leucine-rich-containing family
  • Mitochondria
  • Nucleotide-binding domain
  • Pyrin domain-containing-3

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology

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