Carbohydrate composition of protein and lipid components in sialic acid- rich and -poor low density lipoproteins from subjects with and without coronary artery disease

V. V. Tertov, A. N. Orekhov, I. A. Sobenin, J. D. Morrisett, A. M. Gotto, J. G. Guevara

Research output: Contribution to journalArticle

57 Scopus citations

Abstract

Low density lipoprotein (LDL) from patients with coronary heart disease (CHD) caused 78-286% increase in accumulation of cholesterol in human aortic subendothelial cells compared to 2-17% caused by LDL from normal subjects. Ricin-Sepharose affinity chromatography was used to separate LDL into two subfractions, one sialic acid-rich (SAR) and the other sialic acid-poor (SAP). SAP-LDL from CHD patients caused 156-307% increase in accumulation of cellular cholesterol, whereas SAR-LDL from these patients caused only 14-21% increase. SAP-LDL from normal healthy subjects caused 50-86% increased accumulation, whereas their SAR-LDL induced only 2-12% increase. Carbohydrate analysis of SAP-LDL protein isolated from four CHD patients revealed mean values of 59, 25, 61, and 11 nmoles of N-acetyl glucosamine, galactose, mannose, and sialic acid per mg protein, respectively. Mean values for SAR- LDL protein from these patients were 59, 31, 77, and 24 nmol/mg protein, respectively. Analysis of SAP-LDL protein from four normal healthy subjects indicated respective mean values of 58, 29, 72, and 22 nmol/mg, whereas SAR- LDL protein from normals contained 59, 29, 72, and 29 nmol/mg. The carbohydrate content of LDL lipids represents about 25% of the total carbohydrate present in the lipoprotein. The mean values for SAP-LDL lipids from four CHD patients were about 2, 2, 18, 18, and 2 nmol/mg protein for N- acetyl galactosamine, N-acetyl glucosamine, galactose, glucose, and sialic acid, respectively. The mean values for SAR-LDL lipids from these patients were 3, 4, 34, 41, and 5 nmol/mg, respectively. Analysis of SAP-LDL lipids from four normal healthy subjects indicated respective mean values of 4, 6, 30, 31, and 3 nmol/mg, whereas SAR-LDL lipids from these subjects contained 6, 9, 41, 46, and 7 nmol/mg. These results suggest that the different biological properties of SAR-LDL and SAP-LDL are related to their different carbohydrate compositions.

Original languageEnglish (US)
Pages (from-to)365-375
Number of pages11
JournalJournal of lipid research
Volume34
Issue number3
StatePublished - 1993

Keywords

  • carbohydrate
  • coronary atherosclerosis
  • low density lipoproteins
  • sialic acid

ASJC Scopus subject areas

  • Endocrinology

Fingerprint Dive into the research topics of 'Carbohydrate composition of protein and lipid components in sialic acid- rich and -poor low density lipoproteins from subjects with and without coronary artery disease'. Together they form a unique fingerprint.

Cite this