Canine cardiac sarcoplasmic reticulum is not altered with endurance exercise training

Charlotte Tate, Mary Hamra, Genshun Shin, George Taffet, Perley McBride, Mark Entman

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

To investigate the effect of exercise training on calcium movements by isolated cardiac sarcoplasmic reticulum (SR), mongrel dogs either remained sedentary (S) or were exercise-trained (E) via running for a period of 8-10 wk. The trained state was confirmed by the increase in skeletal muscle citrate synthase activity and decreases in submaximal exercise heart rates in the E group but not in the S dogs. The properties of isolated cardiac SR were identical between the groups. The variables tested included ATP-dependent calcium transport and calcium-stimulated ATPase activity. Importantly, there was no difference in spontaneous calcium release which occurred after peak ATP-dependent calcium accumulation was reached. Calcium release from passively loaded vesicles induced by calcium and ionophore also did not differ in the SR isolated from the E dogs. The change in the affinity of the SR CaATPase for calcium after the addition of the polyanion, heparin, was similar in both groups, indicating that the regulation of calcium-stimulated ATPase activity by the SR protein, phospholamban, is not modified by exercise training. We conclude that exercise training of 8-10 wk duration does not alter the calcium handling properties of cardiac SR isolated from mongrel dogs.

Original languageEnglish (US)
Pages (from-to)1246-1251
Number of pages6
JournalMedicine and science in sports and exercise
Volume25
Issue number11
DOIs
StatePublished - Nov 1993

Keywords

  • CALCIUM RELEASE
  • CALCIUM TRANSPORT
  • HEART
  • PHOSPHOLAMBAN

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Physical Therapy, Sports Therapy and Rehabilitation
  • Orthopedics and Sports Medicine

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