Cancer treatment using an optically inert Rose Bengal derivative combined with pulsed focused ultrasound

Yoo Shin Kim, Valentina Rubio, Jianjun Qi, Rongmin Xia, Zheng Zheng Shi, Leif Peterson, Ching Hsuan Tung, Brian E. O'Neill

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Pulsed high intensity focused ultrasound (HIFU) produced has been combined with a photo-insensitive Rose Bengal derivative (RB2) to provide a synergistic cytotoxicity requiring the presence of both ultrasonic cavitation and drug. In vitro tests have shown that a short treatment (less than 30 s) of pulsed HIFU with peak negative pressure >7 MPa (∼27 W acoustic power at 1.4 MHz) destroys >95 % of breast cancer cells MDA-MB-231 in suspension with >10 μM of the compound. Neither the pulsed HIFU nor the RB2 compound was found to have any significant impact on the viability of the cells when used alone. Introducing an antioxidant (Nacetylcysteine) reduced the effectiveness of the treatment. In vivo tests using these same cells growing as a xenograft in nu/nu mice were also done. An ultrasound contrast agent (Optison) and lower frequency (1.0 MHz) was used to help initiate cavitation at the tumor site. We were able to demonstrate tumor regression with cavitation alone, however, addition of RB2 compound injected i.v. yielded a substantial synergistic improvement over either cavitation or RB2 injection alone.

Original languageEnglish (US)
Title of host publication11th International Symposium on Therapeutic Ultrasound
Pages175-179
Number of pages5
DOIs
StatePublished - 2012
Event11th International Symposium on Therapeutic Ultrasound, ISTU 2011 - New York, NY, United States
Duration: Apr 11 2011Apr 13 2011

Publication series

NameAIP Conference Proceedings
Volume1481
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Other

Other11th International Symposium on Therapeutic Ultrasound, ISTU 2011
CountryUnited States
CityNew York, NY
Period4/11/114/13/11

Keywords

  • Cavitation
  • high intensity ultrasound (HIFU)
  • Optison
  • Rose Bengal
  • sonodynamic therapy

ASJC Scopus subject areas

  • Physics and Astronomy(all)

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