Calcium-dependent binding of uteroglobin (PCB-BP/CCSP) to negatively charged phospholipid liposomes

Magnus Nord; Jan Åke Gustafsson; Johan Lund

doi:10.1016/0014-5793(95)01159-C

Calcium-dependent binding of uteroglobin (PCB-BP/CCSP) to negatively charged phospholipid liposomes

Magnus Nord, Jan Åke Gustafsson, Johan Lund

Houston Methodist

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

To investigate interactions between the polychlorinated biphenyl-binding protein uteroglobin and phospholipids, we used a liposome-pelletting assay. PCB-BP/uteroglobin bound to liposomes made from negatively charged phospholipids (PtdSer and PtdIns) in the presence of 5 mM calcium. No binding to liposomes made from phospholipids without net charge (PtdChol and PtdEtn) was observed, nor could we detect binding in the absence of calcium or when magnesium was substituted for calcium. This suggests that PCB-BP/uteroglobin can bind to phospholipids in vivo and may have a role in the phospholipid homeostasis of the airway and/or secretory pathway of the Clara cell.

Original language	English (US)
Pages (from-to)	403-406
Number of pages	4
Journal	FEBS Letters
Volume	374
Issue number	3
DOIs	https://doi.org/10.1016/0014-5793(95)01159-C
State	Published - Nov 6 1995

Keywords

Calcium
Calcium-binding protein
Carrier protein
Phospholipid
Polychlorinated biphenyl
Uteroglobin

ASJC Scopus subject areas

Structural Biology
Molecular Biology
Genetics
Cell Biology
Biophysics
Biochemistry

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10.1016/0014-5793(95)01159-C

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title = "Calcium-dependent binding of uteroglobin (PCB-BP/CCSP) to negatively charged phospholipid liposomes",

abstract = "To investigate interactions between the polychlorinated biphenyl-binding protein uteroglobin and phospholipids, we used a liposome-pelletting assay. PCB-BP/uteroglobin bound to liposomes made from negatively charged phospholipids (PtdSer and PtdIns) in the presence of 5 mM calcium. No binding to liposomes made from phospholipids without net charge (PtdChol and PtdEtn) was observed, nor could we detect binding in the absence of calcium or when magnesium was substituted for calcium. This suggests that PCB-BP/uteroglobin can bind to phospholipids in vivo and may have a role in the phospholipid homeostasis of the airway and/or secretory pathway of the Clara cell.",

keywords = "Calcium, Calcium-binding protein, Carrier protein, Phospholipid, Polychlorinated biphenyl, Uteroglobin",

author = "Magnus Nord and Gustafsson, {Jan {\AA}ke} and Johan Lund",

note = "Funding Information: Acknowledgements: This work was supported by the Swedish Medical Research Council (Grant 09470) and the research foundations 'Harald Jeanssons stiftelse', 'Harald och Greta Jeanssons stiftelse', 'Stiftelsen Karl Jeppsons minne' and 'Stiftelsen Riksf6rbundet f6r Cystisk Fibros Forskningsfond'. We thank Guntram Suske for providing the coding sequence for human PCB-BP/uteroglobin. We also thank Lena Nordlund-M611er for expert technical assistance, Henry J. Barnes for help with the bacterial expression and Mikael Gillner for fruitful discussions on structure-function relationships of PCB-BP/uteroglobin. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1995",

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doi = "10.1016/0014-5793(95)01159-C",

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pages = "403--406",

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AU - Nord, Magnus

AU - Gustafsson, Jan Åke

AU - Lund, Johan

N1 - Funding Information: Acknowledgements: This work was supported by the Swedish Medical Research Council (Grant 09470) and the research foundations 'Harald Jeanssons stiftelse', 'Harald och Greta Jeanssons stiftelse', 'Stiftelsen Karl Jeppsons minne' and 'Stiftelsen Riksf6rbundet f6r Cystisk Fibros Forskningsfond'. We thank Guntram Suske for providing the coding sequence for human PCB-BP/uteroglobin. We also thank Lena Nordlund-M611er for expert technical assistance, Henry J. Barnes for help with the bacterial expression and Mikael Gillner for fruitful discussions on structure-function relationships of PCB-BP/uteroglobin. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1995/11/6

Y1 - 1995/11/6

N2 - To investigate interactions between the polychlorinated biphenyl-binding protein uteroglobin and phospholipids, we used a liposome-pelletting assay. PCB-BP/uteroglobin bound to liposomes made from negatively charged phospholipids (PtdSer and PtdIns) in the presence of 5 mM calcium. No binding to liposomes made from phospholipids without net charge (PtdChol and PtdEtn) was observed, nor could we detect binding in the absence of calcium or when magnesium was substituted for calcium. This suggests that PCB-BP/uteroglobin can bind to phospholipids in vivo and may have a role in the phospholipid homeostasis of the airway and/or secretory pathway of the Clara cell.

AB - To investigate interactions between the polychlorinated biphenyl-binding protein uteroglobin and phospholipids, we used a liposome-pelletting assay. PCB-BP/uteroglobin bound to liposomes made from negatively charged phospholipids (PtdSer and PtdIns) in the presence of 5 mM calcium. No binding to liposomes made from phospholipids without net charge (PtdChol and PtdEtn) was observed, nor could we detect binding in the absence of calcium or when magnesium was substituted for calcium. This suggests that PCB-BP/uteroglobin can bind to phospholipids in vivo and may have a role in the phospholipid homeostasis of the airway and/or secretory pathway of the Clara cell.

KW - Calcium

KW - Calcium-binding protein

KW - Carrier protein

KW - Phospholipid

KW - Polychlorinated biphenyl

KW - Uteroglobin

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Calcium-dependent binding of uteroglobin (PCB-BP/CCSP) to negatively charged phospholipid liposomes

Abstract

Keywords

ASJC Scopus subject areas

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