Calcium and magnesium competitively influence the growth of a PMR1 deficient Saccharomyces cerevisiae strain

Réka Szigeti, Attila Miseta, Richard Kellermayer

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


PMR1, the Ca2+/Mn2+ ATPase of the secretory pathway in Saccharomyces cerevisiae was the first member of the secretory pathway Ca2+ ATPases (SPCA) to be characterized. In the past few years, pmr1Δ yeast have received more attention due to the recognition that the human homologue of this protein, hSPCA1 is defective in chronic benign pemphigus or Hailey-Hailey disease (HHD). Recent publications have described pmr1Δ S. cerevisiae as a useful model organism for studying the molecular pathology of HHD. Some observations indicated that the high Ca2+ sensitive phenotype of PMR1 defective yeast strains may be the most relevant in this respect. Here we show that the total cellular calcium response of a pmr1Δ S. cerevisiae upon extracellular Ca2+ challenge is decreased compared to the wild type strain similarly as observed in keratinocytes. Additionally, the novel magnesium sensitivity of PMR1 defective yeast is revealed, which appears to be a result of competition for uptake between Ca2+ and Mg2+ at the plasma membrane level. Our findings indicate that extracellular Ca2+ and Mg2+ competitively influence the intracellular Ca2+ homeostasis of S. cerevisiae. These observations may further our understanding of HHD.

Original languageEnglish (US)
Pages (from-to)333-339
Number of pages7
JournalFEMS Microbiology Letters
Issue number2
StatePublished - Oct 15 2005


  • Calcium
  • Hailey-Hailey disease
  • Magnesium
  • PMR1
  • Saccharomyces cerevisiae
  • hSPCA1

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics


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