TY - JOUR
T1 - Calcium and magnesium competitively influence the growth of a PMR1 deficient Saccharomyces cerevisiae strain
AU - Szigeti, Réka
AU - Miseta, Attila
AU - Kellermayer, Richard
N1 - Funding Information:
The authors thank Dr. Rajini Rao and Dr. David M. Bedwell for generously providing strains and plasmids; Ibolya Lamár and Pappné Bácskai Sarolta for their assistance. This work was supported by the Hungarian National Science Fund Grant, OTKA T038144 and the Richter Gedeon Rt for the Hungarian Health Foundation.
PY - 2005/10/15
Y1 - 2005/10/15
N2 - PMR1, the Ca2+/Mn2+ ATPase of the secretory pathway in Saccharomyces cerevisiae was the first member of the secretory pathway Ca2+ ATPases (SPCA) to be characterized. In the past few years, pmr1Δ yeast have received more attention due to the recognition that the human homologue of this protein, hSPCA1 is defective in chronic benign pemphigus or Hailey-Hailey disease (HHD). Recent publications have described pmr1Δ S. cerevisiae as a useful model organism for studying the molecular pathology of HHD. Some observations indicated that the high Ca2+ sensitive phenotype of PMR1 defective yeast strains may be the most relevant in this respect. Here we show that the total cellular calcium response of a pmr1Δ S. cerevisiae upon extracellular Ca2+ challenge is decreased compared to the wild type strain similarly as observed in keratinocytes. Additionally, the novel magnesium sensitivity of PMR1 defective yeast is revealed, which appears to be a result of competition for uptake between Ca2+ and Mg2+ at the plasma membrane level. Our findings indicate that extracellular Ca2+ and Mg2+ competitively influence the intracellular Ca2+ homeostasis of S. cerevisiae. These observations may further our understanding of HHD.
AB - PMR1, the Ca2+/Mn2+ ATPase of the secretory pathway in Saccharomyces cerevisiae was the first member of the secretory pathway Ca2+ ATPases (SPCA) to be characterized. In the past few years, pmr1Δ yeast have received more attention due to the recognition that the human homologue of this protein, hSPCA1 is defective in chronic benign pemphigus or Hailey-Hailey disease (HHD). Recent publications have described pmr1Δ S. cerevisiae as a useful model organism for studying the molecular pathology of HHD. Some observations indicated that the high Ca2+ sensitive phenotype of PMR1 defective yeast strains may be the most relevant in this respect. Here we show that the total cellular calcium response of a pmr1Δ S. cerevisiae upon extracellular Ca2+ challenge is decreased compared to the wild type strain similarly as observed in keratinocytes. Additionally, the novel magnesium sensitivity of PMR1 defective yeast is revealed, which appears to be a result of competition for uptake between Ca2+ and Mg2+ at the plasma membrane level. Our findings indicate that extracellular Ca2+ and Mg2+ competitively influence the intracellular Ca2+ homeostasis of S. cerevisiae. These observations may further our understanding of HHD.
KW - Calcium
KW - Hailey-Hailey disease
KW - Magnesium
KW - PMR1
KW - Saccharomyces cerevisiae
KW - hSPCA1
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U2 - 10.1016/j.femsle.2005.08.017
DO - 10.1016/j.femsle.2005.08.017
M3 - Article
C2 - 16143464
AN - SCOPUS:25644458676
VL - 251
SP - 333
EP - 339
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
SN - 0378-1097
IS - 2
ER -