Brief Communication: P53 Accumulation in N-Methyl-N-Nitrosourea-Induced Rat Mammary Tumors

Keith A. Crist, Raymond D. Fuller, Bina Chaudhuri, Prabir Chaudhuri, You Ming You

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


P53 aberrant protein expression and mutation is a component of many human tumors but less information is available regarding involvement in relevant animal models. We have examined invasive mammary epithelial neoplasms for p53 aberrant protein expression from Sprague-Dawley rats induced by two intrajugular injections of N-methyl-N-nitrosourea (NMU, 50 mg/kg), 7 days apart, beginning at 44-49 days of age. Positive nuclear staining was observed in 8/10 mammary tumor frozen sections using PAb 421, a mouse monoclonal antibody, compared to a mixed mouse IgG negative control antibody. Paraffin sections from two additional sets of similarly induced rat mammary tumors also showed positive nuclear staining (22/37 tumors) with CM-5, a rabbit polyclonal antibody. Our results indicate that elevated cellular content of p53 is a common event in invasive palpable mammary tumors induced by NMU in this dual-injection model system.

Original languageEnglish (US)
Pages (from-to)370-375
Number of pages6
JournalToxicologic Pathology
Issue number3
StatePublished - May 1996


  • Immunohistochemistry
  • adenocarcinoma
  • breast
  • estrus
  • female
  • peroxidase

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Toxicology
  • Cell Biology


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